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NADPH diaphorase activity in peptidergic neurones of the parasitic nematode, Ascaris suum

Published online by Cambridge University Press:  06 April 2009

Z. A. Bascal ,
A. Montgomery ,
L. Holden-Dye ,
R. G. Williams ,
M. C. Thorndyke  and
R. J. Walker
Z. A. Bascal
Affiliation:
Department of Physiology and Pharmacology, Bassett Crescent East, University of Southampton, Southampton SO16 7PX
A. Montgomery
Affiliation:
Department of Physiology and Pharmacology, Bassett Crescent East, University of Southampton, Southampton SO16 7PX
L. Holden-Dye
Affiliation:
Department of Physiology and Pharmacology, Bassett Crescent East, University of Southampton, Southampton SO16 7PX
R. G. Williams
Affiliation:
Department of Physiology and Pharmacology, Bassett Crescent East, University of Southampton, Southampton SO16 7PX
M. C. Thorndyke
Affiliation:
Department of Biology, School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX
R. J. Walker
Affiliation:
Department of Physiology and Pharmacology, Bassett Crescent East, University of Southampton, Southampton SO16 7PX
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Summary

The histochemical marker for nitric oxide synthase, NADPH diaphorase, is known to co-localize in mammalian neurones with various classical neurotransmitters and neuropeptides. The nervous system of the parasitic nematode Ascaris suum has previously been shown to contain both NADPH diaphorase activity and neuropeptide immunoreactivity. This study examined the possibility that NADPH diaphorase and neuropeptide immunoreactivity may co-exist in the same neurones. Two antisera were used, one raised to KYSALMFamide, a C-terminal synthetic analogue of SALMFamide 1 (GFNSALMFamide), and another that recognizes calcitonin-gene-related peptide (CGRP). We provide evidence that in a distinct subset of neurones in the ventral, dorsal and lateral ganglia NADPH diaphorase staining and SALMFamide- like immunoreactivity are co-localized, suggesting a possible role for nitric oxide in modulating neuropeptide activity in these regions. CGRP-like immunoreactivity was less widely distributed, and was not consistently co-localized with NADPH diaphorase.

Keywords

Ascaris suumnematodenitric oxideNADPH diaphoraseCGRPSALMFamideFMRFamide.
Type
Research Article
Information
Parasitology , Volume 112 , Issue 1 , January 1996 , pp. 125 - 134
Copyright
Copyright © Cambridge University Press 1996

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