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MtDNA allows the sensitive detection and haplotyping of the crayfish plague disease agent Aphanomyces astaci showing clues about its origin and migration

Published online by Cambridge University Press:  26 February 2018

Jenny Makkonen ,
Japo Jussila ,
Jörn Panteleit ,
Nina Sophie Keller ,
Anne Schrimpf ,
Kathrin Theissinger ,
Raine Kortet ,
Laura Martín-Torrijos ,
Jose Vladimir Sandoval-Sierra  and
Javier Diéguez-Uribeondo
...Show all authors
Jenny Makkonen*
Affiliation:
Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland
Japo Jussila
Affiliation:
Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland
Jörn Panteleit
Affiliation:
University of Koblenz-Landau, Institute for Environmental Sciences, Fortstrasse 7, D-76829 Landau, Germany
Nina Sophie Keller
Affiliation:
University of Koblenz-Landau, Institute for Environmental Sciences, Fortstrasse 7, D-76829 Landau, Germany Helmholtz-Centre for Environmental Research (UfZ), Department of Isotope Biogeochemistry, Permoserstrasse 15, D-04318 Leipzig, Germany
Anne Schrimpf
Affiliation:
University of Koblenz-Landau, Institute for Environmental Sciences, Fortstrasse 7, D-76829 Landau, Germany
Kathrin Theissinger
Affiliation:
University of Koblenz-Landau, Institute for Environmental Sciences, Fortstrasse 7, D-76829 Landau, Germany
Raine Kortet
Affiliation:
Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 111, FI-80101, Joensuu, Finland
Laura Martín-Torrijos
Affiliation:
Department of Mycology, Real Jardín Botánico CSIC, Plaza de Murillo 2, ES-28014 Madrid, Spain
Jose Vladimir Sandoval-Sierra
Affiliation:
Department of Mycology, Real Jardín Botánico CSIC, Plaza de Murillo 2, ES-28014 Madrid, Spain
Javier Diéguez-Uribeondo
Affiliation:
Department of Mycology, Real Jardín Botánico CSIC, Plaza de Murillo 2, ES-28014 Madrid, Spain
Harri Kokko
Affiliation:
Department of Environmental and Biological Sciences, University of Eastern Finland, P.O. Box 1627, FI-70211, Kuopio, Finland
*
Author for correspondence: Jenny Makkonen, E-mail: [email protected]
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Abstract

The oomycete Aphanomyces astaci, the causative agent of crayfish plague, is listed as one of the 100 worst invasive species in the world, destroying the native crayfish populations throughout Eurasia. The aim of this study was to examine the potential of selected mitochondrial (mt) genes to track the diversity of the crayfish plague pathogen A. astaci. Two sets of primers were developed to amplify the mtDNA of ribosomal rnnS and rnnL subunits. We confirmed two main lineages, with four different haplogroups and five haplotypes among 27 studied A. astaci strains. The haplogroups detected were (1) the A-haplogroup with the a-haplotype strains originating from Orconectes sp., Pacifastacus leniusculus and Astacus astacus; (2) the B-haplogroup with the b-haplotype strains originating from the P. leniusculus; (3) the D-haplogroup with the d1 and d2-haplotypes strains originating from Procambarus clarkii; and (4) the E-haplogroup with the e-haplotype strains originating from the Orconectes limosus. The described markers are stable and reliable and the results are easily repeatable in different laboratories. The present method has high applicability as it allows the detection and characterization of the A. astaci haplotype in acute disease outbreaks in the wild, directly from the infected crayfish tissue samples.

Keywords

Crayfish diseaseInvasive speciesoomyceteribosomal rnnS and rnnL subunitssingle nucleotide polymorphism
Type
Research Article
Information
Parasitology , Volume 145 , Issue 9 , August 2018 , pp. 1210 - 1218
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Copyright
Copyright © Cambridge University Press 2018 

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