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The moving junction protein RON4, although not critical, facilitates host cell invasion and stabilizes MJ members

Published online by Cambridge University Press:  30 June 2017

MING WANG ,
SHINUO CAO ,
NALI DU ,
JIAWEN FU ,
ZHAORAN LI ,
HONGLIN JIA  and
MINGXIN SONG
MING WANG
Affiliation:
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
SHINUO CAO
Affiliation:
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
NALI DU
Affiliation:
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
JIAWEN FU
Affiliation:
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
ZHAORAN LI
Affiliation:
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
HONGLIN JIA*
Affiliation:
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
MINGXIN SONG*
Affiliation:
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China
*
*Corresponding authors: College of Veterinary Medicine, Northeast Agricultural University, Mucai Street 59, Xiangfang District, Harbin 150001, P.R. China. E-mail: [email protected] and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Haping Road 678, Xiangfang District, Harbin 150001, P.R. China. E-mail: [email protected]
*Corresponding authors: College of Veterinary Medicine, Northeast Agricultural University, Mucai Street 59, Xiangfang District, Harbin 150001, P.R. China. E-mail: [email protected] and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Haping Road 678, Xiangfang District, Harbin 150001, P.R. China. E-mail: [email protected]
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Summary

Toxoplasma gondii is an obligate intracellular parasite of phylum Apicomplexa. To facilitate high-efficiency invasion of host cells, T. gondii secretes various proteins related to the moving junction (MJ) complex from rhoptries and micronemes into the interface between the parasite and host. AMA1/RON2/4/5/8 is an important MJ complex, but its mechanism of assembly remains unclear. In this study, we used the CRISPR-Cas9 system to generate a derivative of T. gondii strain RH with a null mutation in TgRON4, thought to be an essential MJ component. Deficiency of TgRON4 moderately decreased invasion ability relative to that of the wild-type parasite. In addition, expression of the endogenous N-terminal fragment of RON5 decreased in the mutant. Together, the results improve our understanding of the assembly mechanism of the MJ complex of T. gondii and raise the possibility of developing new therapeutic drugs that target this complex.

Keywords

TgRON4Toxoplasma gondiiknockoutMJ complex
Type
Research Article
Information
Parasitology , Volume 144 , Issue 11 , September 2017 , pp. 1490 - 1497
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Copyright
Copyright © Cambridge University Press 2017 

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