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A monoclonal antibody to Babesia divergens which inhibits merozoite invasion

Published online by Cambridge University Press:  06 April 2009

Caroline M. Winger ,
Elizabeth U. Canning  and
J. D. Culverhouse
Caroline M. Winger
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB
Elizabeth U. Canning
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB
J. D. Culverhouse
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB
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Summary

A monoclonal antibody-producing hybridoma cell line has been raised against merozoites of Babesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50–60 K by Western blot analysis on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 1 , February 1987 , pp. 17 - 27
Copyright
Copyright © Cambridge University Press 1987

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