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Molecular characterization of Thelastomatoidea (Nematoda: Oxyurida) from cockroaches in Australia

Published online by Cambridge University Press:  27 March 2006

A. R. JEX ,
M. HU ,
H. A. ROSE ,
M. SCHNEIDER ,
T. H. CRIBB  and
R. B. GASSER
A. R. JEX
Affiliation:
School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Queensland, Australia
M. HU
Affiliation:
Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia
H. A. ROSE
Affiliation:
School of Land, Water and Crop Sciences, The University of Sydney, Camperdown, New South Wales, Australia
M. SCHNEIDER
Affiliation:
Department of Zoology and Entomology, The University of Queensland, St Lucia, Queensland, Australia
T. H. CRIBB
Affiliation:
School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Queensland, Australia
R. B. GASSER
Affiliation:
Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia
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Abstract

A molecular approach was used to genetically characterize 5 species (Aoruroides queenslandensis, Blattophila sphaerolaima, Cordonicola gibsoni, Desmicola ornata and Leidynemella fusiformis) belonging to the superfamily Thelastomatoidea (Nematoda: Oxyurida), a group of pinworms that parasitizes terrestrial arthropods. The D3 domain of the large subunit of nuclear ribosomal RNA (LSU) was sequenced for individual specimens, and the analysis of the sequence data allowed the genetic relationships of the 5 species to be studiedNucleotide sequence data reported in this paper are available in the GenBank®, EMBL and DDBJ databases under the Accession numbers: AM232755–AM232763.. The sequence variation in the D3 domain within individual species (0–1·8%) was significantly less than the differences among species (4·3–12·4%). Phylogenetic analyses, using maximum parsimony, maximum likelihood, and neighbour-joining, tree-building methods, established relationships among the 5 species of Thelastomatoidea and Oxyuris equi (a species of the order Oxyurida). The molecular approach employed provides the prospect for developing DNA tools for the specific identification of the Thelastomatoidea, irrespective of developmental stage and sex, as a basis for systematic, ecological and/or population genetic investigations of members within this superfamily.

Keywords

genetic variationThelastomatoideaOxyuridaNematodaD3 domainlarge subunit of nuclear ribosomal RNA
Type
Research Article
Information
Parasitology , Volume 133 , Issue 1 , July 2006 , pp. 123 - 129
Copyright
2006 Cambridge University Press

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References

REFERENCES

Adamson, M. L. ( 1989). Evolutionary biology of the Oxyurida (Nematoda): Biofacies of a haplodiploid taxon. Advances in Parasitology 28, 175–228.CrossRefGoogle Scholar
Adamson, M. L. and Noble, H. ( 1992). Structure of the pinworm (Oxyurida: Nematoda) guild in the hindgut of the American cockroach, Periplaneta americana. Parasitology 104, 497–507.CrossRefGoogle Scholar
Adamson, M. L. and Noble, H. ( 1993). Interspecific and intraspecific competition among pinworms in the hindgut of Periplaneta americana. Journal of Parasitology 79, 50–56.CrossRefGoogle Scholar
Adamson, M. L. and van Waerebeke, D. ( 1992 a). Revision of the Thelastomatoidea, Oxyurida of invertebrate hosts 1. Thelastomatidae. Systematic Parasitology 21, 21–64.Google Scholar
Adamson, M. L. and van Waerebeke, D. ( 1992 b). Revision of the Thelastomatoidea, Oxyurida of invertebrate hosts 2. Travassosinematidae, Protrelloididae and Pseudonymidae. Systematic Parasitology 21, 169–188.Google Scholar
Adamson, M. L. and van Waerebeke, D. ( 1992 c). Revision of the Thelastomatoidea, Oxyurida of invertebrate hosts 3. Hystrignathidae. Systematic Parasitology 22, 111–130.Google Scholar
Blaxter, M., Dorris, M. and De Ley, P. ( 2000). Patterns and processes of evolution of animal parasitic nematodes. Nematology 2, 43–55.CrossRefGoogle Scholar
Blouin, M. S. ( 2002). Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. International Journal for Parasitology 32, 527–531.CrossRefGoogle Scholar
Chilton, N. B., Huby-Chilton, F. and Gasser, R. B. ( 2003). First complete large subunit rRNA sequence and secondary structure for a parasitic nematode – phylogenetic and diagnostic implications. Molecular and Cellular Probes 17, 33–39.CrossRefGoogle Scholar
Chilton, N. B., Gasser, R. B. and Beveridge, I. ( 1995). Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). International Journal for Parasitology 25, 647–651.CrossRefGoogle Scholar
Chitwood, B. G. and Chitwood, M. B. ( 1934). Nematodes parasitic in Philippine cockroaches. Philippine Journal of Science 52, 381–393.Google Scholar
Cobb, N. A. ( 1920). One hundred new nemas (type species of 100 new genera). Contributions to the Science of Nematology, pp. 217–343. Waverly Press, Baltimore.
Connor, S. and Adamson, M. ( 1998). Niche overlap among three species of pinworm parasitic in the hindgut of the American cockroach, Periplaneta americana. Journal of Parasitology 84, 245–247.CrossRefGoogle Scholar
Gasser, R. B., Hu, M., Abs, EL-Osta, Y. G., Zarlenga, D. S. and Pozio, E. ( 2004). Non-isotopic single-strand conformation polymorphism analysis of sequence variability in ribosomal DNA expansion segments within the genus Trichinella (Nematoda: Adenophorea). Electrophoresis 25, 3357–3364.CrossRefGoogle Scholar
Hominick, W. M. and Davey, K. G. ( 1972). The influence of host stage and sex upon the composition of the population of 2 species of thelastomatids parasitic in the hind gut of Periplaneta americana. Canadian Journal of Zoology 50, 945–952.Google Scholar
Hu, M., Chilton, N. B. and Gasser, R. B. ( 2002). Long PCR-based amplification and sequencing of the entire mitochondrial genome from parasitic nematodes. Molecular and Cellular Probes 16, 261–267.CrossRefGoogle Scholar
Iniguez, A. M., Reinhard, K. J., Araujo, A., Ferreira, L. F. and Vicente, A. C. ( 2003). Enterobius vermicularis: ancient DNA from North and South American human coprolites. Memoirs of the Institute of Oswaldo Cruz 98, 67–69.CrossRefGoogle Scholar
Iniguez, A. M., Vicente, A. C., Araujo, A., Ferreira, L. F. and Reinhard, K. J. ( 2002). Enterobius vermicularis: specific detection by amplification of an internal region of 5S ribosomal RNA intergenic spacer and trans-splicing leader RNA analysis. E. vermicularis: specific detection by PCR and SL1 RNA analysis. Experimental Parasitology 102, 218–222.Google Scholar
Jex, A. R., Cribb, T. H. and Schneider, M. A. ( 2004). Aoruroides queenslandensis n. sp. (Oxyurida: Thelastomatoidea), a new nematode from Australian Panesthiinae (Blattodea: Blaberidae). Systematic Parasitology 59, 65–69.Google Scholar
Jex, A. R., Schneider, M. A., Rose, H. A. and Cribb, T. H. ( 2006). The Thelastomatoidea (Nematoda: Oxyurida) of two sympatric Panesthiinae (Blattodea) from south-eastern Queensland, Australia: taxonomy, species richness and host specificity. Nematology 7, 543–575.Google Scholar
Leibersperger, E. ( 1960). Die Oxyuroidea der europaeischen Arthropoden. Parasitologische Schriftenreihe 11, 1–150.Google Scholar
Morand, S. and Rivault, C. ( 1992). Infestation dynamics of Blatticola blattae Graeffe (Nematoda: Thelastomatidae), a parasite of Blattella germanica L. (Dictyoptera: Blattellidae). International Journal for Parasitology 22, 983–989.CrossRefGoogle Scholar
Müller-Graf, C. D., Jobet, E., Cloarec, C., Rivault, C., van Baalen, M. and Morand, S. ( 2001). Population dynamics of host-parasite interactions in a cockroach-oxyurid system. Oikos 95, 431–440.CrossRefGoogle Scholar
Nadler, S. A. and Hudspeth, D. S. ( 1998). Ribosomal DNA and phylogeny of the Ascaridoidea (Nemata: Secernentea): implications for morphological evolution and classification. Molecular Phylogenetics and Evolution 10, 221–236.CrossRefGoogle Scholar
Nadler, S. A., Adams, B. J., Lyons, E. T., DeLong, R. L. and Melin, S. R. ( 2000). Molecular and morphometric evidence for separate species of Uncinaria (Nematoda: Ancylostomatidae) in California sea lions (Zalophus californianus) and northern fur seals (Callorhinus ursinus). Journal of Parasitology 85, 1099–1106.CrossRefGoogle Scholar
Skryabin, K. I., Shikhobalova, N. P. and Mozgovoi, A. A. ( 1984). Key to Parasitic Nematodes: Oxyurata and Ascaridata. E.J. Brill, Leiden.
Swofford, D. L. ( 1999). PAUP*. Sinauer Associates, Sunderland, MA.
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. ( 1997). The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.CrossRefGoogle Scholar
Zervos, S. ( 1988). Evidence for population self-regulation, reproductive competition and arrhenotoky in a thelastomatid nematode of cockroaches. Parasitology 96, 369–379.CrossRefGoogle Scholar