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Molecular characterization of the kinetoplastid membrane protein-11 genes from the parasite Trypanosoma rangeli

Published online by Cambridge University Press:  03 February 2005

H. DIEZ ,
M. C. THOMAS ,
C. P. URUEÑA ,
S. P. SANTANDER ,
C. L. CUERVO ,
M. C. LÓPEZ  and
C. J. PUERTA
H. DIEZ
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad Ciencias, Pontificia Universidad Javeriana, Carrera 7a No 43-82, Edificio 50, Laboratorio 113, Bogotá, Colombia
M. C. THOMAS
Affiliation:
Instituto de Parasitología y Biomedicina López Neyra, CSIC, Avda. del Conocimiento, s/n, Parque Tecnológico de Ciencias de la Salud, 18100-Granada, Spain
C. P. URUEÑA
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad Ciencias, Pontificia Universidad Javeriana, Carrera 7a No 43-82, Edificio 50, Laboratorio 113, Bogotá, Colombia
S. P. SANTANDER
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad Ciencias, Pontificia Universidad Javeriana, Carrera 7a No 43-82, Edificio 50, Laboratorio 113, Bogotá, Colombia
C. L. CUERVO
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad Ciencias, Pontificia Universidad Javeriana, Carrera 7a No 43-82, Edificio 50, Laboratorio 113, Bogotá, Colombia
M. C. LÓPEZ
Affiliation:
Instituto de Parasitología y Biomedicina López Neyra, CSIC, Avda. del Conocimiento, s/n, Parque Tecnológico de Ciencias de la Salud, 18100-Granada, Spain
C. J. PUERTA
Affiliation:
Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad Ciencias, Pontificia Universidad Javeriana, Carrera 7a No 43-82, Edificio 50, Laboratorio 113, Bogotá, Colombia
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Abstract

Trypanosomatids are early divergent parasites which include several species of medical interest. Trypanosoma rangeli is not pathogenic for humans but shows a high immunological cross-reactivity with Trypanosoma cruzi, the causative agent of Chagas' disease that affects more than 17 million people throughout the world. Recent studies have suggested that T. cruzi KMP-11 antigen could be a good candidate for the induction of immunoprotective cytotoxic responses against T. cruzi natural infection. In the present paper the genes coding for the T. rangeli kinetoplastid membrane protein-11 have been characterized. The results show that the locus encoding this protein is formed by 4 gene units measuring 550 nucleotides in length, organized in tandem, and located in different chromosomes in KP1(+) and KP1(−) strains. The gene units are transcribed as a single mRNA of 530 nucleotides in length. Alignment of the T. rangeli KMP-11 deduced amino acid sequence with the homologous KMP-11 protein from T. cruzi revealed an identity of 97%. Interestingly, the T and B cell epitopes of the T. cruzi KMP-11 protein are conserved in the T. rangeli KMP-11 amino acid sequence.

Keywords

Trypanosoma rangeliTrypanosoma cruzikinetoplastid membrane protein-11molecular characterization
Type
Research Article
Information
Parasitology , Volume 130 , Issue 6 , June 2005 , pp. 643 - 651
Copyright
© 2005 Cambridge University Press

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