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The mitochondrial heat shock protein 60 (HSP60) is up-regulated in Onchocerca volvulus after the depletion of Wolbachia

Published online by Cambridge University Press:  21 January 2008

K. M. PFARR ,
U. HEIDER ,
C. SCHMETZ ,
D. W. BÜTTNER  and
A. HOERAUF
K. M. PFARR*
Affiliation:
Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany
U. HEIDER
Affiliation:
Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany
C. SCHMETZ
Affiliation:
Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany
D. W. BÜTTNER
Affiliation:
Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany
A. HOERAUF
Affiliation:
Institute for Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany
*
*Corresponding author: Institute for Medical Parasitology, University of Bonn, Sigmund-Freud-Strasse 25, D-53105, Bonn, Germany. Tel: +49 228 287 11510. Fax: +49 228 287 14330. E-mail: [email protected]
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Summary

Wolbachia, a genus of endosymbiotic bacteria of filarial worms, represent novel targets for anti-filarial therapy. The efficacy of compounds against Wolbachia has been evaluated using antiserum raised against the 60 kDa heat shock protein (HSP60) which binds specifically to this protein in both Wolbachia and mitochondria. It has been shown that Wolbachia stains (using such specific probes) stronger than the mitochondria in untreated Onchocerca volvulus, whereas after the depletion of Wolbachia (with drugs) staining of the mitochondria is increased. Herein, immunogold electron microscopy showed that specific anti-HSP60 serum specifically labelled Wolbachia and filarial mitochondria, and that both have distinct localization patterns, thus allowing them to be differentiated. Immunohistochemistry of O. volvulus showed that HSP60 staining is increased in the mitochondria after Wolbachia depletion in the hypodermis, epithelia, muscles, oocytes, embryos, and developing spermatozoa. This could have been the result of the antiserum preferentially binding to the Wolbachia when they are present or due to increased expression of the protein in the absence of the bacteria. To address this, mRNA levels of filarial hsp60 in O. volvulus were measured. After the depletion of Wolbachia, the transcription of hsp60 was significantly greater (7·7 fold) compared with untreated worms. We hypothesize that the increased expression of HSP60 in the absence of Wolbachia is due to a disruption of the homeostasis of the endosymbiosis.

Keywords

heat-shock protein 60WolbachiafilariaeOnchocerca volvulusmitochondria
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 4 , April 2008 , pp. 529 - 538
Copyright
Copyright © Cambridge University Press 2008

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