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Longitudinal study of Amphibiocystidium sp. infection in a natural population of the Italian stream frog (Rana italica)

Published online by Cambridge University Press:  28 February 2019

Anna Fagotti ,
Roberta Rossi ,
Daniele Canestrelli Open the ORCID record for Daniele Canestrelli [Opens in a new window] ,
Gianandrea La Porta Open the ORCID record for Gianandrea La Porta [Opens in a new window] ,
Romina Paracucchi ,
Livia Lucentini ,
Francesca Simoncelli  and
Ines Di Rosa Open the ORCID record for Ines Di Rosa [Opens in a new window]
Anna Fagotti
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, Perugia, Italy
Roberta Rossi
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, Perugia, Italy
Daniele Canestrelli
Affiliation:
Department of Biological and Ecological Sciences, University of Tuscia, Largo dell'Università s.n.c., Viterbo, Italy
Gianandrea La Porta
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, Perugia, Italy
Romina Paracucchi
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, Perugia, Italy
Livia Lucentini
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, Perugia, Italy
Francesca Simoncelli
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, Perugia, Italy
Ines Di Rosa*
Affiliation:
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, Perugia, Italy
*
Author for correspondence: Ines Di Rosa, E-mail: [email protected]
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Abstract

Mesomycetozoean-induced infections (order Dermocystida, genus Amphibiocystidium) in European and North American amphibians are causing alarm. To date, the pathogenicity of these parasites in field conditions has been poorly studied, and demographic consequences on amphibian populations have not been explored. In this study, an Amphibiocystidium sp. infection is reported in a natural population of the Italian stream frog (Rana italica) of Central Italy, over a 7-year period from 2008 to 2014. Light and electron microscope examinations, as well as partial 18S rDNA sequence analysis were used to characterize the parasite. Moreover, a capture-mark-recapture study was conducted to assess the frog demographics in response to infection. Negative effects of amphibiocystidiosis on individual survival and population fitness were absent throughout the sampling period, despite the high estimates of disease prevalence. This might have been due to resistance and/or tolerance strategies developed by the frogs in response to the persistence of Amphibiocystidium infection in this system. We hypothesized that in the examined R. italica population, amphibiocystidiosis is an ongoing endemic/epidemic infection. However, ecological and host-specific factors, interacting in a synergistic fashion, might be responsible for variations in the susceptibility to Amphibiocystidium infection of both conspecific populations and heterospecific individuals of R. italica.

Keywords

Amphibian declineAmphibiocystidiumemerging infectious diseasemark-recaptureMesomycetozoeaRana italica
Type
Research Article
Information
Parasitology , Volume 146 , Issue 7 , June 2019 , pp. 903 - 910
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Copyright
Copyright © Cambridge University Press 2019 

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