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Leishmania (Viannia) braziliensis-induced chronic granulomatous cutaneous lesions affecting the nasal mucosa in the rhesus monkey (Macaca mulatta) model

Published online by Cambridge University Press:  17 October 2003

A. TEVA ,
R. PORROZZI ,
E. CUPOLILLO ,
C. PIRMEZ ,
M. P. OLIVEIRA-NETO  and
G. GRIMALDI
A. TEVA
Affiliation:
Department of Immunology, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro RJ, CEP 21045-900, Brazil
R. PORROZZI
Affiliation:
Department of Immunology, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro RJ, CEP 21045-900, Brazil Department of Ultrastructural and Cell Biology, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro RJ, CEP 21045-900, Brazil
E. CUPOLILLO
Affiliation:
Department of Immunology, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro RJ, CEP 21045-900, Brazil
C. PIRMEZ
Affiliation:
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro RJ, CEP 21045-900, Brazil
M. P. OLIVEIRA-NETO
Affiliation:
Hospital Evandro Chagas Research Center, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro RJ, CEP 21045-900, Brazil
G. GRIMALDI
Affiliation:
Department of Immunology, FIOCRUZ, Av. Brasil 4365, Rio de Janeiro RJ, CEP 21045-900, Brazil
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Abstract

The present studies on infections with Leishmania (Viannia) braziliensis in rhesus macaques were made to characterize the evolution of different parasite strains and the immune responses they elicited in this experimental host. A standardized inoculum of promastigotes was injected intradermally either above the eyelid or on the forearm of each monkey. Sixteen infected monkeys developed longstanding infections which lasted until the end of the observation period (33 months). The time required for lesion development was very variable, not only for the isolates showing molecular differences but also for individual animals in groups infected with the same parasite strain. The inocula produced lesions of variable severity, ranging from localized cutaneous leishmaniasis (CL) with a tendency to spontaneous healing to non-healing disease. One infected animal developed persistent metastatic skin and mucosal lesions. Anti-Leishmania antibodies and parasite-specific T-cell responses were induced by the experimental infections. As the granulomatous inflammatory response found at the lesions in L. (V.) braziliensis-infected M. mulatta was similar to that in patients with CL, this primate model could be useful for studying the pathophysiology and immunoregulatory events associated with disease evolution, as well as for the evaluation of new drugs or candidate vaccines.

Keywords

mucocutaneous leishmaniasisLeishmania (Viannia) braziliensisPrimateMacaca mulattahistopathology
Type
Research Article
Information
Parasitology , Volume 127 , Issue 5 , November 2003 , pp. 437 - 447
Copyright
2003 Cambridge University Press

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