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Large-scale production of Plasmodium vivax sporozoites

Published online by Cambridge University Press:  06 April 2009

T. Ponnudurai ,
A. H. W. Lensen ,
G. J. van Gemert ,
M. Bolmer ,
A. van Belkum ,
P. van Eerd  and
B. Mons
T. Ponnudurai
Affiliation:
Department of Parasitology, University of Nijmegen, Geert Grooteplein zuid 24, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
A. H. W. Lensen
Affiliation:
Department of Parasitology, University of Nijmegen, Geert Grooteplein zuid 24, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
G. J. van Gemert
Affiliation:
Department of Parasitology, University of Nijmegen, Geert Grooteplein zuid 24, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
M. Bolmer
Affiliation:
Department of Parasitology, University of Nijmegen, Geert Grooteplein zuid 24, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
A. van Belkum
Affiliation:
Primate Center TNO, P.O. Box 5815, 2280 HV Rijswijk, The Netherlands
P. van Eerd
Affiliation:
Primate Center TNO, P.O. Box 5815, 2280 HV Rijswijk, The Netherlands
B. Mons
Affiliation:
Department of Parasitology, University of Leiden, Wassenaarseweg 62, P.O. Box 9605, 2300 RC Leiden, The Netherlands
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Summary

Mass-scale production of Plasmodium vivax sporozoites in Anopheles stephensi was achieved using the chimpanzee (Pan troglodytes) as a source of infective blood. Membrane feeding was as successful as feeding mosquitoes directly on the animal so long as the time between drawing the blood and feeding was restricted to 45 min. Longer delays such as 2–3 h resulted in loss of infectivity in terms of oocyst production. The selected strain of A. stephensi was highly susceptible to P. vivax (Chesson strain). A strain of A. stephensi relatively refractory to P. falciparum showed no cross-refractoriness to P. vivax. Mixed infections of P. falciparum and P. vivax did not interfere with each other in their development in A. stephensi. A second normal blood meal to mosquitoes infected with P. vivax increased the yield of salivary gland sporozoites.

Keywords

Plasmodium vivaxsporozoiteschimpanzeemixed infections
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 317 - 320
Copyright
Copyright © Cambridge University Press 1990

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References

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