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Isolation of Fasciola hepatica haemoglobin

Published online by Cambridge University Press:  06 April 2009

S. McGonigle  and
J. P. Dalton
S. McGonigle
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
J. P. Dalton
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
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Summary

A haemoprotein released in vitro by adult Fasciola hepatica was purified by gel filtration chromatography on Sephacryl S-200 and ion-exchange chromatography on DEAE-Sepharose. The molecule, with an apparent molecular weight of > 200 kDa, contains a haem group and has absorption spectra characteristics similar to haemoglobins. N-terminal amino acid sequence analysis revealed no similarity between the F. hepatica haemoglobin and other vertebrate or invertebrate haemoglobins. Antibodies to the haemoglobin molecule can be detected in the sera of F. hepatica-infected bovines as early as 1 week after infection.

Keywords

liver flukeFasciola hepaticatrematodahaemoproteinhaemoglobin
Type
Research Article
Information
Parasitology , Volume 111 , Issue 2 , August 1995 , pp. 209 - 215
Copyright
Copyright © Cambridge University Press 1995

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