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Isolation and characterization of a putative collagen gene from the potato cyst nematode Globodera pallida

Published online by Cambridge University Press:  06 April 2009

J. T. Jones ,
R. H. Curtis ,
P. J. Wightman  and
P. R. Burrows
J. T. Jones*
Affiliation:
Unit of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland
R. H. Curtis
Affiliation:
Department of Entomology and Nematology, IACR-Rothamsted, Harpenden, Herts AL5 2JQ
P. J. Wightman
Affiliation:
Unit of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland
P. R. Burrows
Affiliation:
Department of Entomology and Nematology, IACR-Rothamsted, Harpenden, Herts AL5 2JQ
*
* Corresponding author: Unit of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland. Tel: 01382 562731. Fax: 01382 563426. E-mail: [email protected].
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Summary

A cDNA clone encoding a full length putative collagen has been isolated in a screen of a mixed stage Globodera pallida expression library. Comparison of the deduced amino acid sequence of this molecule with other collagens suggests it is a cuticular collagen and a member of the col-8 subfamily of collagen genes. Northern blots show the gene is expressed specifically in gravid, adult females of the parasite as compared to second (invasive) stage juveniles and virgin females. Preliminary immunocytochemical studies indicate this collagen is present in areas other than the cuticle; these findings and the potential functional role of this collagen are discussed.

Keywords

nematodecollagenGlobodera pallidaDNA
Type
Research Article
Information
Parasitology , Volume 113 , Issue 6 , December 1996 , pp. 581 - 588
Copyright
Copyright © Cambridge University Press 1996

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