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Isoenzyme characterization of trypanosomes of the subgenus Herpetosoma

Published online by Cambridge University Press:  06 April 2009

H. A. Mohamed ,
D. H. Molyneux  and
C. M. Scott
H. A. Mohamed
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
D. H. Molyneux
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
C. M. Scott
Affiliation:
Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans
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Summary

Isoenzyme analysis was used to characterize 6 species of trypanosomes of the subgenus Herpetosoma using 13 different enzyme systems. The species studied were Trypanosoma lewisi, T. musculi, T. grosi, T. microti, T. evotomys and T. nabiasi which cannot be distinguished on morphological grounds. Extracts for thin-layer starch-gel electrophoresis were prepared from cultures of insect forms in either Schneider's Drosophila or Grace's insect tissue culture media with foetal calf serum or a nutrient agar medium. Extracts of T. lewisi and T. musculi bloodstream forms were also run for comparison. All parasites gave distinct patterns which enabled them to be differentiated on one or more enzyme systems. Two types of computer analysis were used to group the parasites; using these techniques the murine parasites T. lewisi, T. musculi and T. grosi fell into one broad group, and T. microti and T. evotomys of microtine rodents formed another. These findings are in accord with earlier observations on the behavioural characteristics of these parasites in their mammalian host and their vector (fleas). The clear differences observed provide the basis for the application of other biochemical and immunological techniques for differentiation within this subgenus of trypanosomes.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 1 , February 1987 , pp. 39 - 48
Copyright
Copyright © Cambridge University Press 1987

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