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Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis

Published online by Cambridge University Press:  29 August 2012

H. T. GONZAGA ,
C. VILA-VERDE ,
D. S. NUNES ,
V. S. RIBEIRO ,
J. P. CUNHA-JÚNIOR  and
J. M. COSTA-CRUZ
H. T. GONZAGA
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
C. VILA-VERDE
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
D. S. NUNES
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
V. S. RIBEIRO
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
J. P. CUNHA-JÚNIOR
Affiliation:
Laboratório de Imunoquímica e Imunotecnologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
J. M. COSTA-CRUZ*
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
*
*Corresponding author: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil, Avenida Pará 1720, 38400-902, Uberlândia, MG, Brasil. Tel: +55 34 3218 2187. Fax: +55 34 3118 2333. E-mail: [email protected]
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Summary

The aim of this study was to fractionate and partially characterize the antigenic extract of filariform larvae of Strongyloides venezuelensis in ion-exchange resin diethylaminoethyl sepharose (DEAE), to obtain antigenic fractions potentially applicable in immunoassays. Somatic antigen (SA) and its fractions DEAE S1 and DEAE S2 - which interacted with the resin - were evaluated by 1-dimensional electrophoresis to obtain protein profiles. SA and its fractions were tested in serum samples for IgG detection by ELISA. Serum samples (n = 155) were analysed: 50 from strongyloidiasis patients (G1), 55 from patients with other parasitic infections (G2) and 50 from healthy volunteers. Sensitivity (Se), specificity (Sp), area under curve (AUC) and likelihood ratios (LR) were calculated. The DEAE S2 fraction provided a high diagnostic value for IgG detection (Se 92·0%, Sp 91·4%, AUC 0·981, LR+ 10·75, LR − 0·09). In conclusion, the DEAE S2 fraction would probably be a source of immunodominant polypeptides for IgG detection in human strongyloidiasis serodiagnosis.

Keywords

strongyloidiasisserodiagnosision exchangeheterologous antigens
Type
Research Article
Information
Parasitology , Volume 140 , Issue 1 , January 2013 , pp. 69 - 75
Copyright
Copyright © Cambridge University Press 2012

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