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Interferon-γ induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases

Published online by Cambridge University Press:  01 March 2000

M. HAMADIEN ,
M. BAKHIET  and
R. A. HARRIS
M. HAMADIEN
Affiliation:
Division for Infectious Diseases, Huddinge University Hospital, SE-141 86 Huddinge
M. BAKHIET
Affiliation:
Division for Infectious Diseases, Huddinge University Hospital, SE-141 86 Huddinge
R. A. HARRIS
Affiliation:
Neuroimmunology Unit, Centre for Molecular Medicine, Karolinska Hospital, S-171 76 Stockholm, Sweden
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Abstract

Bidirectional signalling between Trypanosoma brucei brucei and CD8+ T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-γ. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-γ in a dose–response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-γ-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.

Keywords

Trypanosoma bruceiIFN-γtrypanosome-derived lymphocyte triggering factor (TLTF)
Type
Research Article
Information
Parasitology , Volume 120 , Issue 3 , March 2000 , pp. 281 - 287
Copyright
2000 Cambridge University Press

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