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Influence of variation in host strain and parasite isolate on inflammatory and antibody responses to Trichinella spiralis in mice

Published online by Cambridge University Press:  06 April 2009

P. K. Goyal  and
D. Wakelin
P. K. Goyal
Affiliation:
MRC Experimental Parasitology Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD
D. Wakelin*
Affiliation:
MRC Experimental Parasitology Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD
*
*Reprint requests to Professor D. Wakelin.
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Summary

Variation in the immunogenicity of 3 isolates of Trichinella spiralis was assessed by the parameters of adult worm recovery, mast cell, eosinophil and antibody responses in mice of defined response phenotype. The levels of the protective, inflammatory and immune responses induced by infection differed between the isolates. Isolates showed considerable variation in the capacity to elicit mast cell and eosinophil responses. All induced increases in parasite-specific antibody, levels of total (IgGAM) antibody and of IgM and IgG isotypes rose steadily after infection, but there were significant differences in levels of response. The IgGAM response was correlated with the number of worms present, i.e. the greatest response was seen in low responder (C57BL/10) mice infected with the longest-surviving isolates. All isolates elicited specific IgG1 and IgG2a antibodies after infection, although, again, there were isolate-specific differences in the levels and kinetics of response. Levels of these isotypes were always higher, although not significantly so, in high-responder NIH mice. Low-responder mice showed higher IgE serum levels than high-responder mice after infection, one isolate giving much higher IgE values than the other two.

Keywords

immunityTrichinella spiralismicehost variationantibody responsesinflammatory responses.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 4 , May 1993 , pp. 371 - 378
Copyright
Copyright © Cambridge University Press 1993

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