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Increased susceptibility to Strongyloides venezuelensis in mice due to Mycobacterium bovis co-infection which modulates production of Th2 cytokines

Published online by Cambridge University Press:  07 August 2009

A. M. CARMO ,
M. A. VICENTINI ,
A. T. DIAS ,
L. L. ALVES ,
C. C. S. ALVES ,
J. S. BRANDI ,
M. L. DE PAULA ,
A. FERNANDES ,
M. M. BARSANTE  and
M. A. SOUZA
...Show all authors
A. M. CARMO
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
M. A. VICENTINI
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
A. T. DIAS
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
L. L. ALVES
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
C. C. S. ALVES
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
J. S. BRANDI
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
M. L. DE PAULA
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
A. FERNANDES
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil
M. M. BARSANTE
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
M. A. SOUZA
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
H. C. TEIXEIRA
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
D. NEGRÃO-CORRÊA
Affiliation:
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil
A. P. FERREIRA*
Affiliation:
Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-330, Minas Gerais, Brazil
*
*Corresponding author: Laboratório de Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Campus Martelos, 36036-330 Juiz de Fora, MG, Brazil. Tel: +55 32 32293214. Fax: +55 32 32293214. E-mail address: [email protected].
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Summary

An estimated quarter of the world's population possesses an infection caused by gastrointestinal nematodes, which induce a Th2 type immune response. Concomitant infection of nematodes with Mycobacterium tuberculosis, which induces a predominantly Th1 type response, is very frequent in tropical and subtropical regions. This study examined immune responses of BALB/c mice infected with Strongyloides venezuelensis and then co-infected with Mycobacterium bovis. The number of worms in the intestine, eggs in feces, cytokine production in lungs and intestine and the expression of CD80, CD86, CTLA-4 and CD28 cell markers on pulmonary cells were analysed. Our results indicate that co-infected mice had an increased parasite burden, which correlates with elevated IFN-γ and IL-10 cytokine production and decreased IL-4 and IL-13. Moreover, decreased expression of CD80 and increased expression of CTLA-4 were observed in co-infected mice. Our data point out that susceptibility to Strongyloides venezuelensis infection is increased by Mycobacterium bovis co-infection, resulting in higher parasite survival.

Keywords

Mycobacterium bovisStrongyloides venezuelensisco-infectionBALB/c micecytokine productionparasite susceptibility
Type
Research Article
Information
Parasitology , Volume 136 , Issue 11 , September 2009 , pp. 1357 - 1365
Copyright
Copyright © Cambridge University Press 2009

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