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In vitro compatibility and nematicidal activity of Monacrosporium sinense and Pochonia chlamydosporia for biological control of bovine parasitic nematodes

Published online by Cambridge University Press:  26 April 2021

Isabela de Castro Oliveira Open the ORCID record for Isabela de Castro Oliveira [Opens in a new window] ,
Ítalo Stoupa Vieira Open the ORCID record for Ítalo Stoupa Vieira [Opens in a new window] ,
Artur Kanadani Campos  and
Jackson Victor de Araújo
Isabela de Castro Oliveira*
Affiliation:
Veterinary Department, Laboratory of Parasitology and Parasitic Diseases, Federal University of Viçosa, Av. PH Holfs, s/n, CEP 36570-900, Viçosa, Minas Gerais, Brazil
Ítalo Stoupa Vieira
Affiliation:
Veterinary Department, Laboratory of Parasitology and Parasitic Diseases, Federal University of Viçosa, Av. PH Holfs, s/n, CEP 36570-900, Viçosa, Minas Gerais, Brazil
Artur Kanadani Campos
Affiliation:
Veterinary Department, Laboratory of Parasitology and Parasitic Diseases, Federal University of Viçosa, Av. PH Holfs, s/n, CEP 36570-900, Viçosa, Minas Gerais, Brazil
Jackson Victor de Araújo
Affiliation:
Veterinary Department, Laboratory of Parasitology and Parasitic Diseases, Federal University of Viçosa, Av. PH Holfs, s/n, CEP 36570-900, Viçosa, Minas Gerais, Brazil
*
Author for correspondence: Isabela de Castro Oliveira, E-mail: [email protected]
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Abstract

The use of nematophagous fungi is an alternative for the biological control of nematodes in ruminants. In this study, the compatibility of joint growth of the fungi Monacrosporium sinense and Pochonia chlamydosporia and the joint nematicidal activity of these fungal isolates on bovine infective larvae were evaluated. For that, tests of direct confrontation, the effect of volatile compounds and antibiosis were conducted. In order to carry out the tests, the fungi were inoculated in potato dextrose agar culture medium and, after the incubation period, the growth of the colonies, the formation of an inhibition halo and the effect of volatile metabolites were verified. The compatibility between fungi isolates M. sinense and P. chlamydosporia was confirmed and the nematicidal evaluation proved the best effectiveness was when both were used together, with a 98.90% reduction in the number of bovine nematode infective larvae under in vitro conditions. It was concluded that M. sinense and P. chlamydosporia presented synergistic action, suggesting that the joint application of the fungi increases the effectiveness of biological control of bovine infective larvae.

Keywords

Antibiosisbiocontroldirect confrontationhelminthsinteractionsvolatile metabolites
Type
Research Article
Information
Parasitology , Volume 148 , Issue 8 , July 2021 , pp. 956 - 961
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Avramenko, RW, Redman, EM, Windeyer, C and Gilleard, JS (2020) Assessing anthelmintic resistance risk in the post-genomic era: a proof-of-concept study assessing the potential for widespread benzimidazole-resistant gastrointestinal nematodes in North American cattle and bison. Parasitology 147, 897–906.CrossRefGoogle ScholarPubMed
Ayupe, TH, Monteiro, TSA, Braga, FR, Soares, FEF, Mello, INK, Araujo, JM, Freitas, LG and Araújo, JV (2016) Assessment of compatibility between the nematophagous fungi Arthrobotrys robusta and Duddingtonia flagrans under laboratory conditions. Revista Iberoamericana de Micología 33, 129–130.CrossRefGoogle Scholar
Baker, D, Rice, S, Leemon, D, Godwin, R and James, P (2020) Development of a mycoinsecticide bait formulation for the control of house flies, Musca domestica L. Insects 11, 47.CrossRefGoogle ScholarPubMed
Bell, DK, Wells, HD, Markhabell, DK and Wells, CR (1982) In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology 72, 379–382.CrossRefGoogle Scholar
Bharat, R, Singh, VN and Singh, DB (1980) Trichoderma viride as a mycoparasite of Aspergillus spp. Plant and Soil 57, 131–135.Google Scholar
Braga, F and Araújo, JV (2014) Nematophagous fungi for biological control of gastrointestinal nematodes in domestic animals. Applied Microbiology and Biotechnology 98, 71–82.CrossRefGoogle ScholarPubMed
Campos, AK, Araújo, JV, Assis, RCL, Gandra, JR and Guimarães, MP (2007) Viabilidade de formulação peletizada do fungo nematófago Monacrosporium sinense, no controle biológico de nematóides parasitos gastrintestinais de bezerros. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 59, 14–20.CrossRefGoogle Scholar
Carnevali, F, Franchini, D, Otranto, D, Giangaspero, A, Di Bello, A, Ciccarelli, S, Szpila, K, Valastro, C and Van der Esch, AS (2019) A formulation of neem and hypericum oily extract for the treatment of the wound myiasis by Wohlfahrtia magnifica in domestic animals. Parasitology Research 118, 2361–2367.CrossRefGoogle ScholarPubMed
Charlier, J, Rinaldi, L, Musella, V, Ploeger, HW, Chartier, C, Vineer, HR, Hinney, B, von Samson-Himmelstjerna, G, Băcescu, B, Mickiewicz, M, Mateus, TL, Martinez-Valladares, M, Quealy, S, Azaizeh, H, Sekovska, B, Akkari, H, Petkevicius, S, Hektoen, L and Claerebout, E (2020) Initial assessment of the economic burden of major parasitic helminth infections to the ruminant livestock industry in Europe. Preventive Veterinary Medicine 182, 105103.CrossRefGoogle ScholarPubMed
Dallemole-Giaretta, R, Freitas, LG, Cavallin, IC, Marmentini, GA, Faria, CMR and Resende, JTV (2013) Evaluation of a Pochonia chlamydosporia based product, for the control of Meloidogyne javanica in culture and in carrot field. Nematropica 43, 131–137.Google Scholar
Dias-Filho, MB (2016) Uso de Pastagens para a Produção de Bovinos de Corte no Brasil: Passado, Presente e Futuro. In Dias-Filho, MB (ed.), Embrapa Amazônia Oriental. Belém, PA: Documentos, pp. 1–42.Google Scholar
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) (2019) Available at https://www.embrapa.br/macrologistica/producao-agropecuaria (Accessed 12 November 2019).Google Scholar
Keith, RK (1953) The differentiation on the infective larvae of some common nematode parasites of cattle. Australian Journal of Zoology 1, 223–235.CrossRefGoogle Scholar
Lackey, BA, Muldoon, AE and Jaffe, BA (1993) Alginate pellet formulation of Hirsutella rossiliensis for biological control of plant-parasitic nematodes. Biology Control 3, 155–160.CrossRefGoogle Scholar
Mendoza-de-Gives, P, Davies, KG, Clark, SJ and Behnke, JM (1999) Predatory behavior of trapping fungi against srf mutants of Caenorhabditis elegans and different plant and animal parasitic nematódeos. Parasitology 119, 95–104.CrossRefGoogle Scholar
Ministério da Agricultura Pecuária e Abastecimento (MAPA) (2019) Available at http://www.agricultura.gov.br/insumospecuarios/produtosveterinarios. Relação de produtos farmacêuticos e biológicos que tiveram suas solicitações de registro deferidas no ano de 2019 (Accessed 11 March 2019).Google Scholar
Mukhtar, T and Pervaz, I (2003) In vitro evaluation of ovicidal and larvicidal effects of culture filtrate of Verticillium chlamydosporium against Meloidogyne javanica. International Journal of Agriculture and Biology 5, 576–579. Available at http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.319.239&rep=rep1&type=pdf (Accessed 15 March 2021).Google Scholar
Oliveira, IC, Carvalho, LM, Vieira, IS, Campos, AK, Freitas, SG, Araujo, JM, Braga, FR and Araújo, JV (2018) Using the fungus Arthrobotrys cladodes var. macroides as a sustainable strategy to reduce numbers of infective larvae of bovine gastrointestinal parasitic nematódeos. Journal of Invertebrate Pathology 158, 46–51.CrossRefGoogle Scholar
Szwec, M, De Waal, T and Zintl, A (2021) Biological methods for the control of gastrointestinal nematodes. The Veterinary Journal 268, 105602.CrossRefGoogle Scholar
Van Ooij, C (2011) Fungal pathogenesis: hungry fungus eats nematode. Nature Reviews Microbiology 9, 766–767.CrossRefGoogle ScholarPubMed
Velde, FV, Charlier, J and Claerebout, E (2018) Farmer behavior and gastrointestinal nematodes in ruminant livestock – uptake of sustainable control approaches. Frontiers in Veterinary Science 5, 255.CrossRefGoogle Scholar
Vieira, ÍS, Oliveira, IC, Campos, AK and Araújo, JV (2019) Association and predatory capacity of fungi Pochonia chlamydosporia and Arthrobotrys cladodes in the biological control of parasitic helminths of bovines. Parasitology 146, 1347–1351.CrossRefGoogle ScholarPubMed
Vieira, ÍS, Oliveira, IC, Campos, AK and Araújo, JV (2020a) In vitro biological control of bovine parasitic nematodes by Arthrobotrys cladodes, Duddingtonia flagrans and Pochonia chlamydosporia under different temperature conditions. Journal of Helminthology 94, 1–6.CrossRefGoogle Scholar
Vieira, ÍS, Oliveira, IC, Freitas, SG, Campos, AK and Araújo, JV (2020b) Arthrobotrys cladodes and Pochonia chlamydosporia in the biological control of nematodosis in extensive bovine production system. Parasitology 147, 699–705.CrossRefGoogle Scholar
Walker, HL and Connick, WJ (1983) Sodium alginate for production and formulation of mycoherbicides. Weed Science 31, 333–338. Available at https://www.jstor.org/stable/4043716.CrossRefGoogle Scholar
Yang, J, Liang, L, Li, J and Zhang, KQ (2013) Nematicidal enzymes from microorganisms and their applications. Applied Microbiology and Biotechnology 97, 7081–7095.CrossRefGoogle ScholarPubMed
Yuan, X, Zheng, J, Jiao, S, Cheng, G, Feng, C, Du, Y and Liu, H (2019) A review on the preparation of chitosan oligosaccharides and application to human health, animal husbandry and agricultural production. Carbohydrate Polymers 220, 60–70.CrossRefGoogle ScholarPubMed