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Immunological and pathological responses in BALB/c mice induced by genetic administration of Tc13 Tul antigen of Trypanosoma cruzi

Published online by Cambridge University Press:  15 February 2006

G. A. GARCÍA ,
M. R. ARNAIZ ,
S. A. LAUCELLA ,
M. I. ESTEVA ,
N. AINCIART ,
A. RIARTE ,
P. A. GARAVAGLIA ,
L. E. FICHERA  and
A. M. RUIZ
G. A. GARCÍA
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
M. R. ARNAIZ
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
S. A. LAUCELLA
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
M. I. ESTEVA
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
N. AINCIART
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina Present address: Instituto Leloir, Av. Patricias Argentinas 435, Buenos Aires, Argentina.
A. RIARTE
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
P. A. GARAVAGLIA
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
L. E. FICHERA
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
A. M. RUIZ
Affiliation:
Insituto Nacional de Parasitología ‘Dr. Mario Fatala Chabén’. Paseo Colón 568. (1063) Buenos Aires, Argentina
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Abstract

Tc13 is a trans-sialidase family protein of Trypanosoma cruzi, the aetiological agent of Chagas' disease. Recently, in vitro studies had suggested that Tc13 might participate in the pathogenesis of the disease. In order to study the role of Tc13 antigens in an in vivo model, we administered plasmid DNA encoding a Tc13 antigen from the Tulahuén strain (Tc13 Tul) to BALB/c mice and evaluated the immunological and pathological manifestations as well as the capacity of this antigen to confer protection against T. cruzi infection. Tc13 Tul immunization did not elicit a detectable humoral immune response but induced specific memory T-cells with no capacity to produce IFN-γ. Five months after DNA-immunization with Tc13 Tul, signs of hepatotoxicity and reactive changes in the heart, liver and spleen were observed in 40–80% of mice. When Tc13 Tul DNA-immunized animals were challenged with trypomastigotes, a significant decrease in parasitaemia in early and late acute phase was observed without modification in the survival rate. Surprisingly, Tc13 Tul-immunized mice chronically infected with T. cruzi showed a decrease in the severity of heart damage. We conclude that, in BALB/c mice, genetic immunization with Tc13 Tul mainly induces immune responses associated with pathology.

Keywords

Trypanosoma cruzigenetic immunizationTc13myocarditisChagas disease
Type
Research Article
Information
Parasitology , Volume 132 , Issue 6 , June 2006 , pp. 855 - 866
Copyright
2006 Cambridge University Press

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