Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-30T23:14:53.675Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunoglobulin-G binding proteins in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus

Published online by Cambridge University Press:  06 April 2009

H. Wang  and
P. A. Nuttall
H. Wang
Affiliation:
NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
P. A. Nuttall
Affiliation:
NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
Get access Rights & Permissions [Opens in a new window]

Summary

A guinea-pig immunoglobulin-G linked agarose column was used to detect the immunoglobulin-G binding proteins (IGBP) in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus. IGBPs were found in salivary gland extract (SGE) of the unfed ticks of all 3 species. The sizes of the IGBPs, as determined by SDS–polyacrylamide gel electrophoresis, differed between species. In R. appendiculatus 2 IGBP bands were detected in haemolymph collected from unfed females and males, and an additional 78 kDa IGBP was detected in haemolymph samples of male and female ticks that had fed for 6 days. Although there was no difference in the IGBP profile of SGE derived from unfed male and female R. appendiculatus, 2 new IGBPs were detected in the SGE of the male ticks after they had fed for 6 days. The possible function of IGBP in ticks is discussed.

Keywords

immunoglobulin binding proteintick salivary glandtick haemolymphRhipicephalus appendiculatusAmblyomma variegatumIxodes hexagonus
Type
Research Article
Information
Parasitology , Volume 111 , Issue 2 , August 1995 , pp. 161 - 165
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ackerman, S., Clare, F. B., McGill, T. W. & Sonenshine, D. E. (1981). Passage of host serum components, including antibody, across the digestive tract of Dermacentor variabilis (Say). Journal of Parasitology 67, 737–40.CrossRefGoogle ScholarPubMed
Allen, J. R. & Humphreys, S. J. (1979). Immunisation of guinea pigs and cattle against ticks. Nature, London 280, 491–3.CrossRefGoogle ScholarPubMed
Azad, A. F., Vaughan, J. A. & Wirtz, R. A. (1989). Host anti–parasite IgG: transport across the gut epithelial lining, and its influence on the infectivity of arthropod vectors. In Host Regulated Developmental Mechanisms in Vector Arthropods, (ed. Borovsky, D. & Spielman, A.) pp. 163–170. Florida, USA: University of Florida IFAS.Google Scholar
Ben-Yakir, D., Fox, J. C., Homer, J. T. & Barker, R. W. (1986). Quantitative studies of host immunoglobulin G passage into the haemocoel of the tick Amblyomma americanum and Dermacentor variabilis. In Morphology, Physiology and Behavioral Biology of Ticks, (ed. Sauer, J. R. & Hair, J. A.) pp. 329–339. Chichester: Ellis Horwood Ltd.Google Scholar
Brossard, M. & Rais, O. (1984). Passage of haemolysins through the midgut epithelium of female Ixodes ricinus L. fed on rabbits infected or reinfected with ticks. Experientia 40, 561–2.CrossRefGoogle ScholarPubMed
Brown, S. J. & Askenase, P. W. (1986). Amblyomma americanum: physiochemical isolation of a protein derived from the salivary gland that is capable of inducing immune resistance in guinea pigs. Experimental Parasitology 62, 40–50.CrossRefGoogle ScholarPubMed
Chinzei, Y. & Minoura, H. (1987). Host immunoglobin G titre and antibody activity in haemolymph of the tick, Ornithodoros moubata. Medical and Veterinary Entomology 1, 409–16.CrossRefGoogle Scholar
Fujisaki, K., Kamio, T. & Kitaoka, S. (1984). Passage of host serum components, including antibodies specific for Theileria sergenti, across the digestive tract of argasid and ixodid ticks. Annals of Tropical Medicine and Parasitology 78, 449–50.CrossRefGoogle ScholarPubMed
Jones, L. D. & Nuttall, P. A. (1989). The effect of virusimmune hosts on Thogoto virus infection of the tick, Rhipicephalus appendiculatus. Virus Research 14, 129–40.CrossRefGoogle ScholarPubMed
Lackie, A. M. & Gavin, S. (1989). Uptake and persistence of ingested antibody in the mosquito Anopheles stephensi. Medical and Veterinary Entomology 3, 225–30.CrossRefGoogle ScholarPubMed
Sauer, J. R., McSwain, J. L. & Essenberg, R. C. (1994). Cell membrane receptors and regulation of cell function in ticks and blood-sucking insects. International Journal for Parasitology 24, 33–52.CrossRefGoogle ScholarPubMed
Wang, H. & Nuttall, P. A. (1994 a). Excretion of host immunoglobulin in tick saliva and detection of IgG-binding protein in tick haemolymph and salivary gland. Parasitology 109, 525–30.CrossRefGoogle Scholar
Wang, H. & Nuttall, P. A. (1994 b). Comparison of the proteins in salivary glands, saliva, and haemolymph of Rhipicephalus appendiculatus female ticks during feeding. Parasitology 109, 517–23.CrossRefGoogle ScholarPubMed