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Immunogenicity of a hybrid Plasmodium falciparum, malaria antigen

Published online by Cambridge University Press:  06 April 2009

M. J. Lockyer ,
H. Cooper ,
J. Tite ,
W. Rowan  and
J. S. Crowe
M. J. Lockyer
Affiliation:
Department of Cell Biology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
H. Cooper
Affiliation:
Department of Cell Biology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
J. Tite
Affiliation:
Department of Cell Biology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
W. Rowan
Affiliation:
Department of Cell Biology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
J. S. Crowe
Affiliation:
Department of Cell Biology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
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Summary

A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of the antibody response to the CSP repeats was not overcome by the PMMSA component, the response to which showed no restriction. In an adjuvant trial the highest antibody titres in rabbits to both components of the hybrid were obtained using Freund's adjuvant. Lack of a boosting antibody response to the CSP repeats appeared to be linked to the conformation of the PMMSA component. Formulation of the hybrid protein into Iscoms gave antibody titres of only short duration to both components.

Keywords

baculovirushybrid antigencircumsporozoite proteinmalariaantigensadjuvantsIscoms
Type
Research Article
Information
Parasitology , Volume 106 , Issue 5 , June 1993 , pp. 451 - 457
Copyright
Copyright © Cambridge University Press 1993

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