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Identification of the avian tracheal trematode Typhlocoelum cucumerinum (Trematoda: Cyclocoelidae) in a host–parasite–environment system: diagnosis, life cycle and molecular phylogeny

Published online by Cambridge University Press:  09 June 2021

Jordana C. A. Assis Open the ORCID record for Jordana C. A. Assis [Opens in a new window] ,
Danimar López-Hernández ,
Samantha Favoretto ,
Lilian B. Medeiros ,
Alan L. Melo ,
Nelson R. S. Martins  and
Hudson A. Pinto
Jordana C. A. Assis*
Affiliation:
Laboratório de Biologia de Trematoda, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Danimar López-Hernández
Affiliation:
Laboratório de Biologia de Trematoda, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Samantha Favoretto
Affiliation:
Department of Veterinary Medicine, Universidade Federal de Lavras, Lavras, Brazil
Lilian B. Medeiros
Affiliation:
Laboratório de Doenças das Aves, Department of Preventive Veterinary Medicine, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Alan L. Melo
Affiliation:
Laboratório de Biologia de Trematoda, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Nelson R. S. Martins
Affiliation:
Laboratório de Doenças das Aves, Department of Preventive Veterinary Medicine, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Hudson A. Pinto
Affiliation:
Laboratório de Biologia de Trematoda, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
*
Author for correspondence: Jordana C. A. Assis, E-mail: [email protected]
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Abstract

Typhlocoelum cucumerinum is a tracheal parasite of birds widely distributed across the globe. Nevertheless, aspects of the biology of this cyclocoelid are still poorly understood. Herein, we report the finding of T. cucumerinum in definitive and intermediate hosts from an urban waterbody of Brazil. The parasite was initially detected during the necropsy of domestic Muscovy ducks (Cairina moschata) found dead in the locality. Coproparasitological tests in live animals revealed that 12/47 (25.53%) Muscovy ducks and 2/8 (25%) mallards (Anas platyrhynchos platyrhynchos) were infected with T. cucumerinum. Moreover, rediae and metacercariae morphologically similar to T. cucumerinum were found in 3/248 (1.33%) Biomphalaria straminea collected in the same waterbody frequented by the birds. The conspecificity between the adult and the larval stages was confirmed molecularly (100% similarity in Cox-1). Moreover, the phylogenetic position of T. cucumerinum was determined for the first time based on partial fragments of the 28S, Cox-1 and Nad-1 genes. The species grouped with other members of the subfamily Typhlocoelinae with sequences available, but the data obtained do not support the distinctiveness of the genera Typhlocoelum and Tracheophilus. Further studies involving a broader range of species can result in taxonomic rearrangements in Typhlocoelinae.

Keywords

Typhlocoelinaelife cycledomestic waterfowlphylogeny
Type
Research Article
Information
Parasitology , Volume 148 , Issue 11 , September 2021 , pp. 1383 - 1391
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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