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Genetic studies on Plasmodium chabaudi: recombination between enzyme markers

Published online by Cambridge University Press:  06 April 2009

D. Walliker ,
R. Carter  and
A. Sanderson
D. Walliker
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
R. Carter
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
A. Sanderson
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
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Extract

Two lines of Plasmodium chabaudi differing in three characters have been crossed, using a technique previously described for P. yoelii. One line, termed 47AS, was characterized by an electrophoretic form of 6-phosphogluconate dehydrogenase, denoted 6PGD-2, a form of lactate dehydrogenase, denoted LDH-3, and was pyrimethamine-resistant. The second line, termed 10AJ, possessed enzyme forms 6PGD-3 and LDH-2 and was pyrimethamine-sensitive. The cross was made by permitting mosquitoes to feed on a mixture of the two lines and infecting rodents with the resulting sporozoites. The products of the cross were cloned by dilution and examined for enzyme-type and drug-response. Results showed that recombination had occurred between each of the three characters. Clones characterized by 6PGD-2/LDH-2 and 6PGD-3/LDH-3 demonstrated recombination between the enzyme markers. The drug-resistance character segregated independently of either enzyme marker.

Type
Research Article
Information
Parasitology , Volume 70 , Issue 1 , February 1975 , pp. 19 - 24
Copyright
Copyright © Cambridge University Press 1975

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References

Carter, R., (1973). Enzyme variation in Plasmodium berghei and Plasmodium vinckei. Parasitology 66, 297–307.Google Scholar
Carter, R., & Walliker, D., (1975). New observations on the malaria parasites of rodents of the Central African Republic; Plasmodium vinckei petteri subsp.nov. and Plasmodium chabaudi Landau, 1965. Annals of Tropical Medicine and Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Killick-Kendrick, R., (1974). Parasitic protozoa of the blood of rodents. II. Haemogregarines, malaria parasites and piroplasms of rodents: an annotated checklist and host index. Acta tropica, Separatum vol. 31 (1), 28.Google Scholar
Lumsden, W. H. R., Cunningham, M. P., Webber, W. A. F., van Hoeve, K., Knight, R. H., & Simmons, V., (1965). Some effects of hydrogen ion concentration on trypanosome numbers and infectivity. Experimental Parasitology 16, 8–17.CrossRefGoogle ScholarPubMed
Oxbrow, A. I., (1973). Strain specific immunity to Plasmodium berghei: a new genetic marker. Parasitology 67, 17–27.CrossRefGoogle ScholarPubMed
Sinden, R. E., & Canning, E. U., (1973). Ultrastructure and cytochemistry of nuclear division in Plasmodium. Progress in Protozoology, p. 385. (Abstract of papers read at the Fourth International Congress of Protozoology, Clermont-Ferrand, 1973.)Google Scholar
Walliker, D., (1972). An infection of Plasmodium berghei derived from sporozoites of a single oocyst. Transactions of the Royal Society of Tropical Medicine and Hygiene 4, 543.Google Scholar
Walliker, D., Carter, R., & Morgan, S., (1971). Genetic recombination in malaria parasites. Nature, London 232, 561–2.Google Scholar
Walliker, D., Carter, R., & Morgan, S., (1973). Genetic recombination in Plasmodium berghei. Parasitology 66, 309–320.Google Scholar
Yoeli, M., Upmanis, R. S., & Most, H., (1969). Drug-resistance transfer among rodent plasmodia. 1. Acquisition of resistance to pyrimethamine by a drug-sensitive strain of Plasmodium berghei in the course of its concomitant development with a pyrimethamine-resistant P. vinckei strain. Parasitology 59, 429–47.CrossRefGoogle ScholarPubMed