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Fluctuations in rat liver alanine-amino-transferase activity during experimental nippostrongylosis

Published online by Cambridge University Press:  06 April 2009

Lucy J. Robertson
Lucy J. Robertson
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
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Summary

The activity of the gluconeogenic enzyme, alanine-amino-transferase (ALT), in a preparation from the liver of rats was studied by means of an in vitro assay throughout the course of a primary infection of Nippostrongylus brasiliensis, established by a subcutaneous injection of approximately 4000 3rd-stage larvae. The activity was measured on days 1–14 p.i. in both uninfected and infected rats and a marked pattern in the enzyme's activity was observed. In infected rats, the activity increased from 1·46±0·19 U/g liver on day 1 p.i. to a peak on day 4 p.i. of 10·75±1·62 U/g liver, then decreased to a trough of 0·44±0·18 U/g liver on day 10 p.i. before returning to original levels by day 14 p.i., by which time the infection had been largely eliminated. In uninfected rats the activity of the liver enzyme remained constant throughout this period with a value of 2·54±0·12 U/g liver. The activity of the enzyme in vitro was found to be related to the size of the inoculum on days 4 and 10 p.i. It was proposed that these observations could be due to either (1) a direct effect of the parasite, or (2) a consequence of the host immune response to the infection. In order to investigate the second proposition more fully, liver ALT activity was investigated by in vitro assay on selected days p.i. in rats experiencing a secondary N. brasiliensis infection. In these rats the liver ALT activity was observed to reach a peak on day 2 p.i., with an activity of 3·87 ± 0-28 U/g liver, and a trough on day 4 p.i. with an activity of 0·11 ±0·03 U/g liver, returning to similar levels to those measured in uninfected rats by day 7 p.i. When serum prepared from rats having secondary N. brasiliensis infections collected on day 4 p.i. was added to the assay, a reduction in the activity of liver ALT activity from both the infected and uninfected rats was measured by in vitro assay. The results are discussed in relation to protein metabolism and gluconeogenesis in rats infected with N. brasiliensis, and also in relation to the host’s immune response to the parasitic infection.

Keywords

alanine-amino-transferaseNippostrongylus brasiliensisgluconeogenesisimmune response
Type
Research Article
Information
Parasitology , Volume 98 , Issue 2 , April 1989 , pp. 301 - 306
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Ash, C. P. J., Crompton, D. W. T. & Lunn, P. G. (1985). Endocrine responses of protein-malnourished rats infected with Nippostrongylus brasiliensis (Nematoda). Parasitology 91, 359–68.Google Scholar
Beisel, W. R. (1972). Interrelated changes of host metabolism during generalised infectious illness. American Journal of Clinical Nutrition 25, 1254–60.Google Scholar
Caldwell, E. F. & Mchenry, E. W. (1953). Studies on vitamin B6 and transamination in rat liver. Archives of Biochemistry and Biophysics 45, 97–104.CrossRefGoogle ScholarPubMed
Crompton, D. W. T., Arnold, S., Coward, W. A. & Lunn, P. G. (1978). Nippostrongylus (Nematoda) infection in protein malnourished rats. Transactions of the Royal Society of Tropical Medicine and Hygeine 72, 195–7.CrossRefGoogle ScholarPubMed
Crompton, D. W. T., Walters, D. E. & Arnold, S. (1981). Changes in the food intake and body weight of protein malnourished rats infected with Nippostrongylus brasiliensis (Nematoda). Parasitology 82, 23–8.Google Scholar
Felig, P. (1973). The glucose-alanine cycle. Metabolism 22, 179–207.Google Scholar
Felig, P., Pozefsky, T., Marliss, E. & Cahill, G. F. Jr. (1970). Alanine: Key role in gluconeogenesis. Science 167, 1003–4.CrossRefGoogle ScholarPubMed
Gavosto, F., Pileri, A. & Brusca, A. (1957). Increased transaminase activity in the liver after administration of cortisone. Biochimica et Biophysica Acta 24, 250–4.Google Scholar
Keymer, A., Crompton, D. W. T. & Sahakian, B. J. (1983). Parasite-induced learned taste aversion involving Nippostrongylus in rats. Parasitology 86, 455–60.Google Scholar
Long, C. L. (1977). Energy balance and carbohydrate metabolism in infection and sepsis. In Proceedings of a Workshop: Impact of Infection on Nutritional Status of the Host. American Journal of Clinical Nutrition 30, 1301–10.Google Scholar
Lunn, P. G., Northrop, C. A. & Wainwright, M. (1988). Hypoalbuminemia in energy-malnourished rats infected with Nippostrongylus brasiliensis (Nematoda). Journal of Nutrition 118, 121–7.CrossRefGoogle ScholarPubMed
Nawa, Y. (1979). Increased permeability of gut mucosa in rats infected with Nippostrongylus brasiliensis. International Journal for Parasitology 9, 251–5.Google ScholarPubMed
Nolla, H., Bristol, J. R. & Mayberry, L. F. (1985). Nippostrongylus brasiliensis: malabsorption in experimentally infected rats. Experimental Parasitology 39, 180–4.CrossRefGoogle Scholar
Ovington, K. S. (1985 a). Physiological and metabolic responses of the rat to Nippostrongylus brasiliensis infection. Ph.D. thesis, University of Cambridge.Google Scholar
Ovington, K. S. (1985 b). Dose-dependent relationships between Nippostrongylus brasiliensis populations and rat food intake. Parasitology 91, 157–67.CrossRefGoogle ScholarPubMed
Rosen, F., Roberts, N. R., Budwick, L E. & Nichol, C. A. (1958). An enzymatic basis for the gluconeogenic action of hydrocortisone. Science 127, 287–8.CrossRefGoogle Scholar
Rosen, F., Roberts, N. R. & Nichol, C. A. (1958). Glucocorticosteroids and transaminase activity. I. Increased activity of glutamic-pyruvic-transaminase in four conditions associated with gluconeogenesis. Journal of Biological Chemistry 234, 476–80.CrossRefGoogle Scholar
Segal, H. L., Beattie, D. S. & Hopper, S. (1962). Purification and properties of liver glutamic-alanine transaminase from normal and corticoid treated rats. Journal of Biological Chemistry 237, 1914–20.CrossRefGoogle Scholar
Segal, H. L., Rosso, R. G., Hopper, S. & Weber, M. M. (1963). Direct evidence for an increase in enzyme level as the basis of the glucocorticoid induced increase in glutamic-alanine transaminase activity in rat liver. Journal of Biochemical Chemistry 237, PC3303–PC3305.Google Scholar
Sitren, H. S. & Stevenson, N. R. (1978). The effects of meal feeding at different times of day on daily changes in serum insulin, gastrin and liver enzymes in the rat. Journal of Nutrition 108, 1393–1401.CrossRefGoogle ScholarPubMed
Wannemacher, R. W. (1977). Key role of various individual amino acids in host response to infection. American Journal of Clinical Nutrition 30, 1269–80.Google Scholar
Wannemacher, R. W., Neufeld, H. A. & Canonico, R. G. (1976). Hepatic gluconeogenic capacity and rate during pneumococcal infection in rats. Federation Proceedings 35, 343.Google Scholar