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Fibronectin degradation as biomarker for Trypanosoma cruzi infection and treatment monitoring in mice

Published online by Cambridge University Press:  24 May 2021

Nora Adriana Hernández-Cuevas Open the ORCID record for Nora Adriana Hernández-Cuevas [Opens in a new window] ,
Andrea Marín-Cervera ,
Shineily Garcia-Polanco ,
Pedro Martínez-Vega Open the ORCID record for Pedro Martínez-Vega [Opens in a new window] ,
Miguel Rosado-Vallado  and
Eric Dumonteil
Nora Adriana Hernández-Cuevas*
Affiliation:
Laboratorio de Parasitología, Centro de Investigaciones Regionales ‘Dr. Hideyo Noguchi’, Universidad Autónoma de Yucatán, Mérida, México
Andrea Marín-Cervera
Affiliation:
Laboratorio de Parasitología, Centro de Investigaciones Regionales ‘Dr. Hideyo Noguchi’, Universidad Autónoma de Yucatán, Mérida, México
Shineily Garcia-Polanco
Affiliation:
Laboratorio de Parasitología, Centro de Investigaciones Regionales ‘Dr. Hideyo Noguchi’, Universidad Autónoma de Yucatán, Mérida, México
Pedro Martínez-Vega
Affiliation:
Laboratorio de Parasitología, Centro de Investigaciones Regionales ‘Dr. Hideyo Noguchi’, Universidad Autónoma de Yucatán, Mérida, México
Miguel Rosado-Vallado
Affiliation:
Laboratorio de Parasitología, Centro de Investigaciones Regionales ‘Dr. Hideyo Noguchi’, Universidad Autónoma de Yucatán, Mérida, México
Eric Dumonteil
Affiliation:
Department of Tropical Medicine, School of Public Health and Tropical Medicine, and Vector-Borne and Infectious Disease Research Center, Tulane University, New Orleans, LA, USA
*
Author for correspondence: Nora Adriana Hernández-Cuevas, E-mail: [email protected]
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Abstract

Biomarkers (coming from host or parasite) to monitor Chagas disease (CD) progression as well as the therapeutic response in chronic CD are critically needed, since seronegativization, which may be considered the best indicator of therapeutic cure, takes several years to be observed in adults. Several molecules have been suggested as biomarkers for CD, however, they have to be validated. Taking advantage of mouse models of Trypanosoma cruzi infection, we investigated changes in the degradation profile of fibronectin in plasma. The degradation profile of fibronectin was different in the acute phase compared to the chronic phase of the infection. Fibronectin fragments of approximately 150, 100, 40 and 30 kDa were identified. Furthermore, those degradation profiles correlated with acute parasitaemia as well as with cardiac parasite burden and tissue damage during the infection. The usefulness of fibronectin degradation as a biomarker for therapeutic response following drug treatment and immunotherapeutic vaccination also was evaluated and a decreased fibronectin degradation profile was observed upon benznidazole or a vaccine candidate treatment.

Keywords

Acute and chronic infectionbiomarkerChagas diseasefibronectinmouse modeltreatment
Type
Research Article
Information
Parasitology , Volume 148 , Issue 9 , August 2021 , pp. 1067 - 1073
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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