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Evaluation of the immunodiagnostic potential of a recombinant surface protein domain from Acanthamoeba castellanii

Published online by Cambridge University Press:  19 July 2016

ALEMAO G. CARPINTEYRO SÁNCHEZ ,
VERIDIANA GOMES VIRGINIO ,
VINICIUS JOSÉ MASCHIO ,
HENRIQUE BUNSELMEYER FERREIRA  and
MARILISE BRITTES ROTT
ALEMAO G. CARPINTEYRO SÁNCHEZ
Affiliation:
Programa de Pós-graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciencias Básicas e da Saúde, UFRGS, Porto Alegre, RS, Brazil
VERIDIANA GOMES VIRGINIO
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil
VINICIUS JOSÉ MASCHIO
Affiliation:
Programa de Pós-graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciencias Básicas e da Saúde, UFRGS, Porto Alegre, RS, Brazil
HENRIQUE BUNSELMEYER FERREIRA
Affiliation:
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, UFRGS, Porto Alegre, RS, Brazil
MARILISE BRITTES ROTT*
Affiliation:
Programa de Pós-graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciencias Básicas e da Saúde, UFRGS, Porto Alegre, RS, Brazil Departamento de Parasitologia, Instituto de Ciências Basicas da Saúde, UFRGS, Porto Alegre, RS, Brazil
*
*Corresponding author: Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, RS, Porto Alegre, Brazil. E-mail: [email protected]
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Summary

Acanthamoeba spp. are free-living protists widely distributed in environment, able to cause keratitis, encephalitis and skin lesions in humans and animals. Acanthamoeba spp. exist in two forms: an infective trophozoite and a dormant cyst. Several factors contribute to the pathogenesis of Acanthamoeba spp. The parasite adhesion to the host cell is the primary step for infection and is mediated by a mannose binding-protein, expressed in the surface and considered the main pathogenicity factor in Acanthamoeba spp. So far, there was no evidence of another surface protein of Acanthamoeba spp. relevant for host invasion or infection by these organisms. The aims of this study were to identify and characterize an Acanthamoeba castellanii surface protein and to evaluate its diagnostic potential. In silico predictions of surface proteins allowed to identify the A. castellanii calreticulin as a possible surface antigen. The coding sequence of a predicted extracellular domain of A. castellanii calreticulin was cloned by in vivo homologous recombination and the recombinant polypeptide (AcCRT29–130) was produced. Its immunodiagnostic potential was assessed in a recombinant antigen-based ELISA with sera from experimentally infected rats that developed keratitis and encephalitis, and sera from patients with encephalitis. The AcCRT29–130 was significantly more recognized by sera from encephalitis infected rats in comparison with the non-infected controls. Human sera from encephalitis patients, however presented no significant response. These results showed the AcCRT29–130 potential for A. castellanii infection immunodiagnosis in animals, with further studies being required for assessment of its use for human infections.

Keywords

Acanthamoeba castellaniicalreticulinrecombinant proteinimmunodiagnosticELISA
Type
Research Article
Information
Parasitology , Volume 143 , Issue 12 , October 2016 , pp. 1656 - 1664
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Copyright
Copyright © Cambridge University Press 2016 

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