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Effects of Cryptocaryon irritans infection on the survival, feeding, respiratory rate and ionic regulation of the marbled rockfish Sebastiscus marmoratus

Published online by Cambridge University Press:  21 October 2013

FEI YIN ,
QIYANG GONG ,
YANWEI LI ,
XUEMING DAN ,
PENG SUN ,
QUANXIN GAO ,
ZHAOHONG SHI ,
SHIMING PENG  and
ANXING LI
FEI YIN
Affiliation:
Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, People's Republic of China
QIYANG GONG
Affiliation:
Key Laboratory for Aquatic Products Safety of Ministry of Education, State Key Laboratory of Biocontrol, The School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street,Haizhu District, Guangzhou, Guangdong Province 510275, People's Republic of China
YANWEI LI
Affiliation:
Key Laboratory for Aquatic Products Safety of Ministry of Education, State Key Laboratory of Biocontrol, The School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street,Haizhu District, Guangzhou, Guangdong Province 510275, People's Republic of China
XUEMING DAN
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China
PENG SUN
Affiliation:
Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, People's Republic of China
QUANXIN GAO
Affiliation:
Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, People's Republic of China
ZHAOHONG SHI
Affiliation:
Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, People's Republic of China
SHIMING PENG
Affiliation:
Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, People's Republic of China
ANXING LI*
Affiliation:
Key Laboratory for Aquatic Products Safety of Ministry of Education, State Key Laboratory of Biocontrol, The School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street,Haizhu District, Guangzhou, Guangdong Province 510275, People's Republic of China
*
* Corresponding author: Key Laboratory for Aquatic Products Safety of Ministry of Education, State Key Laboratory of Biocontrol, The School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street, Haizhu District, Guangzhou, Guangdong Province 510275, People's Republic of China. E-mail: [email protected]
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Summary

To clarify the effects of a Cryptocaryon irritans infection on the physiological functions of the marbled rockfish Sebastiscus marmoratus, this study utilized C. irritans at concentrations of 2500; 5000; 7500; 10 000; 20 000; and 30 000 theronts/fish to infect marbled rockfish weighing 45±3 g. The survival rate, food intake, respiratory rate, serum ion concentrations and gill Na+/K+-ATPase activity were determined. With the increase of the infection concentration and the passage of time, the survival rate of the rockfish gradually decreased. The groups infected with more than 5000 theronts/fish had stopped feeding within 4 days. The respiratory rates of the fish in the groups infected with 2500 and 5000 theronts/fish initially increased and then decreased. In contrast, the respiratory rate of the fish in the groups infected with more than 7500 theronts/fish was elevated to levels significantly higher than the control group after 12 h. The Na+/K+-ATPase activity and serum Na+ and Cl− concentrations increased with increasing infection concentration. In conclusion, the physiological functions of the fish infected with low concentrations of C. irritans can be effectively restored, whereas a high concentration infection induced severe stress. The declined food intake and accelerated respiratory rate could be useful for an early warning system as important indicators.

Keywords

Cryptocaryon irritansSebastiscus marmoratussurvivalfood intakerespiratory rateion contentNa+/K+-ATPase activity
Type
Research Article
Information
Parasitology , Volume 141 , Issue 2 , February 2014 , pp. 279 - 286
Copyright
Copyright © Cambridge University Press 2013 

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References

REFERENCES

Affonso, E. G. and Rantin, F. T. (2005). Respiratory responses of the air-breathing fish Hoplosternum littorale to hypoxia and hydrogen sulfide. Comparative Biochemistry and Physiology C–Toxicology and Pharmacology 141, 275–280. doi: 10.1016/j.cca.2005.07.003.Google Scholar
Aguado Giménez, F. and García García, B. (2002). Growth and food intake models in Octopus vulgaris Cuvier (1797): influence of body weight, temperature, sex and diet. Aquaculture International 10, 361–377. doi: 10.1023/a:1023335024053.Google Scholar
Bai, J. S., Xie, M. Q., Zhu, X. Q., Dan, X. M. and Li, A. X. (2008). Comparative studies on the immunogenicity of theronts, tomonts and trophonts of Cryptocaryon irritans in grouper. Parasitology Research 102, 307–313. doi: 10.1007/s00436-007-0766-6.Google Scholar
Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Burgess, P. J. and Matthews, R. A. (1995). Fish host range of seven isolates of Cryptocaryon irritans (Ciliophora). Journal of Fish Biology 46, 727–729. doi: 10.1111/j.1095-8649.1995.tb01109.x.Google Scholar
Cerezo Valverde, J., Martínez López, F. J. and García García, B. (2006). Oxygen consumption and ventilatory frequency responses to gradual hypoxia in common dentex (Dentex dentex): basis for suitable oxygen level estimations. Aquaculture 256, 542–551. doi: 10.1016/j.aquaculture.2006.02.030.Google Scholar
Chen, A. P., Jiang, Y. L., Qian, D., Chen, C. F., Li, A. X., Huang, J. and Yang, B. (2011). Cryptocaryoniasis. China Fisheries 8, 39–40.Google Scholar
Cheung, P. J., Nigrelli, R. F. and Ruggieri, G. D. (1979). Studies on cryptocaryoniasis in marine fish: effect of temperature and salinity on the reproductive cycle of Cryptocaryon irritans Brown, 1951. Journal of Fish Diseases 2, 93–97. doi: 10.1111/j.1365-2761.1979.tb00146.x.Google Scholar
Colorni, A. (1985). Aspects of the biology of Cryptocaryon irritans, and hyposalinity as a control measure in cultured gilt-head sea bream Sparus aurata . Diseases of Aquatic Organisms 1, 19–22.Google Scholar
Colorni, A. and Burgess, P. (1997). Cryptocaryon irritans Brown 1951, the cause of ‘white spot disease’ in marine fish: an update. Aquarium Sciences and Conservation 1, 217–238. doi: 10.1023/a:1018360323287.CrossRefGoogle Scholar
Dan, X. M., Li, A. X., Lin, X. T., Teng, N. and Zhu, X. Q. (2006). A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus . Aquaculture 258, 127–133. doi: 10.1016/j.aquaculture.2006.04.026.Google Scholar
Diggles, B. K. and Adlard, R. D. (1997). Intraspecific variation in Cryptocaryon irritans . Journal of Eukaryotic Microbiology 44, 25–32. doi: 10.1111/j.1550-7408.1997.tb05686.x.Google Scholar
Huff, J. A. and Burns, C. D. (1981). Hypersaline and chemical control of Cryptocaryon irritans in red snapper, Lutjanus campechanus, monoculture. Aquaculture 22, 181–184. doi: 10.1016/0044-8486(81)90144-7.Google Scholar
Kawano, F., Hirazawa, N., Gravningen, K. and Berntsen, J. O. (2012). Antiparasitic effect of dietary Romet®30 (SDMX–OMP) against ciliate Cryptocaryon irritans infection in the red sea bream Pagrus major and tiger puffer Takifugu rubripes . Aquaculture 344–349, 35–39. doi: 10.1016/j.aquaculture.2012.02.028.CrossRefGoogle Scholar
Li, Y. W., Dan, X. M., Zhang, T. W., Luo, X. C. and Li, A. X. (2011 a). Immune-related genes expression profile in orange-spotted grouper during exposure to Cryptocaryon irritans . Parasite Immunology 33, 679–987. doi: 10.1111/j.1365-3024.2011.01337.x.Google Scholar
Li, Y. W., Luo, X. C., Dan, X. M., Huang, X. Z., Qiao, W., Zhong, Z. P. and Li, A. X. (2011 b). Orange-spotted grouper (Epinephelus coioides) TLR2, MyD88 and IL-1[beta] involved in anti-Cryptocaryon irritans response. Fish and Shellfish Immunology 30, 1230–1240. doi: 10.1016/j.fsi.2011.04.012.Google Scholar
Lin, C. H., Tsai, R. S. and Lee, T. H. (2004). Expression and distribution of Na, K-ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge. Comparative Biochemistry and Physiology A–Molecular and Integrative Physiology 138, 287–295. doi: 10.1016/j.cbpb.2004.04.005.Google Scholar
Liu, Z. Y., Lin, X. J., Xie, Y. Q., Wu, N. J. and Fan, X. J. (2012). Research on death caused by secondary bacterial infection of Cryptocaryon irritans on Pseudosciaena crocea . Journal of Fujian Fisheries 34, 11–15.Google Scholar
Luo, X. C., Xie, M. Q., Zhu, X. Q. and Li, A. X. (2007). Protective immunity in grouper (Epinephelus coioides) following exposure to or injection with Cryptocaryon irritans . Fish and Shellfish Immunology 22, 427–432. doi: 10.1016/j.fsi.2006.04.011.Google Scholar
Matthews, R. A. and Burgess, P. J. (1995). Cryptocaryon irritans (Ciliophora): primary infection in thick-lipped mullet, Chelon labrosus (Risso). Journal of Fish Diseases 18, 329–335. doi: 10.1111/j.1365-2761.1995.tb00309.x.Google Scholar
Misumi, I. (2009). The ciliated protozoan parasite, Cryptocaryon irritans, and protective immunity in marine fish. The University of Hawai'i, Hawai'i, USA.Google Scholar
Misumi, I., Leong, J.-A., Takemura, A. and Lewis, T. (2012). Immune protection of Mozambique tilapia (Oreochromis mossambicus) exposed to different infectious doses of ectoparasite (Cryptocaryon irritans). Parasitology Research 110, 363–372. doi: 10.1007/s00436-011-2500-7.Google Scholar
Peng, S. M., Yin, F., Shi, Z. H., Sun, P., Wang, J. G. and Liu, Z. Y. (2011). Optimum water temperature for the growth of juvenile common Chinese cuttlefish, Sepiella maindroni (De Rochebrune 1884). Journal of Shellfish Research 30, 205–209. doi: 10.2983/035.030.0202.Google Scholar
Sun, Z. Y., Zheng, C. F., Wu, X. Y., Guo, G. W., Wang, Y. Y. and Huang, X. H. (2011). The strain and life-cycle of Cryptocaryon irritans isolated from Pseudosciaena crocea cultured in Xiapu, Fujian. Journal Fujian Normal University (Natural Science Edition) 27, 101–108.Google Scholar
Wang, F. H., Xie, M. Q. and Li, A. X. (2010). A novel protein isolated from the serum of rabbitfish (Siganus oramin) is lethal to Cryptocaryon irritans . Fish and Shellfish Immunology 29, 32–41. doi: 10.1016/j.fsi.2010.01.006.Google Scholar
Wang, F. H., Li, R. J., Xie, M. Q. and Li, A. X. (2011). The serum of rabbitfish (Siganus oramin) has antimicrobial activity to some pathogenic organisms and a novel serum L-amino acid oxidase is isolated. Fish and Shellfish Immunology 30, 1095–1108. doi: 10.1016/j.fsi.2011.02.004.Google Scholar
Xu, R. L., Jiang, J. B. and Cheng, B. S. (1992). Light microscopy observation on the life cycle of the Cryptocaryon irritans . Marine Sciences 3, 42–44.Google Scholar
Yan, M. C., Shao, X. B., Shan, L. Z. and Xie, Q. L. (2008). Study on prevention and treatment of Cryptocaryon irritans with formalin for brown croaker, Miichthys miiuy . Modern Fisheries Information 23, 16–19.Google Scholar
Yoshinaga, T., Im, H. J., Nishida, S. and Ogawa, K. (2011). In vitro and in vivo efficacies of ionophores against Cryptocaryon irritans . Aquaculture 321, 167–172. doi: 10.1016/j.aquaculture.2011.08.028.Google Scholar