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A dipeptidylpeptidase secreted by Fasciola hepatica

Published online by Cambridge University Press:  06 April 2009

C. Carmona ,
S. McGonigle ,
A. J. Dowd ,
A. M. Smith ,
S. Coughlan ,
E. McGowran  and
J. P. Dalton
C. Carmona
Affiliation:
Unidad de Biologia Parasitaria, Instituto de Higiene, Universidad de la República, Montevideo, Uruguay
S. McGonigle
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
A. J. Dowd
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
A. M. Smith
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
S. Coughlan
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
E. McGowran
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
J. P. Dalton
Affiliation:
School of Biological Sciences, Dublin City University, Dublin 9, Republic of Ireland
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Summary

A dipeptidylpeptidase (DPP) was isolated from Fasciola hepatica by gel-filtration and ion-exchange chromatography. The exoproteinase is secreted by newly excysted juveniles, immature and mature flukes. The liver fluke DPP is a serine proteinase of molecular weight > 200 kDa and differs from previously characterized mammalian DPPs in its substrate preference and susceptibility to inactivation by inhibitors. The parasite DPP may function in the latter stages of the proteolytic digestion of host macromolecules. In this manner, the enzyme may be important in providing the parasite with dipeptides that could be absorbed through the intestine as nutrient.

Keywords

liver flukeFasciola hepaticaTrematodadipeptidylpeptidaseexopeptidase
Type
Research Article
Information
Parasitology , Volume 109 , Issue 1 , July 1994 , pp. 113 - 118
Copyright
Copyright © Cambridge University Press 1994

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References

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