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Differential diagnosis of Taenia saginata and Taenia solium with DNA probes

Published online by Cambridge University Press:  06 April 2009

L. J. S. Harrison ,
J. Delgado  and
R. M. E. Parkhouse
L. J. S. Harrison
Affiliation:
Centre for Tropical Vetrinary Medicine, Department of Tropical Animal HealthUniversity of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG
J. Delgado
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
R. M. E. Parkhouse
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

A size selected genomic DNA library was constructed using DNA extracted from Taenia saginata. The DNA was digested using the restriction enzyme EcoR1 under star conditions and the 2–4 kbase fraction, selected following sucrose density-gradient separation, was cloned in the bacteriophage λgt 10. A panel of cestode DNAs including Taenia saginata, Taenia solium, Taenia taeniaeformis, Taenia crassiceps, Echinococcus granulosus and DNAs of bovine, porcine and human origin were used in conjunction with hybridization analysis to identify two recombinant bacteriophages. The first probe, designated HDP1, reacted specifically with T. saginata DNA. The second, designated HDP2, reacted with DNA from both T. saginata and T. solium but not the other DNA samples and thus provided a general reagent for positive identification of fragments of Taenia spp. proglottides of human faecal origin. If used in conjunction the two DNA probes allow positive identification of T. saginata. In the clinical situation it is important to be able to distinguish T. saginata and T. solium infections and DNA probes such as these may be useful in such differentiation.

Keywords

Taenia saginataTaenia solium differentiationDNA probeshybridization
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 459 - 461
Copyright
Copyright © Cambridge University Press 1990

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References

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