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Diagnosis of toxoplasmosis in pregnancy. Evaluation of latex–protein complexes by immnunoagglutination

Published online by Cambridge University Press:  14 March 2017

LEANDRO E. PERETTI ,
VERÓNICA D. G. GONZALEZ ,
IVÁN S. MARCIPAR  and
LUIS M. GUGLIOTTA
LEANDRO E. PERETTI
Affiliation:
INTEC (Universidad Nacional del Litoral and CONICET), Santa Fe (3000), Argentina
VERÓNICA D. G. GONZALEZ
Affiliation:
INTEC (Universidad Nacional del Litoral and CONICET), Santa Fe (3000), Argentina
IVÁN S. MARCIPAR
Affiliation:
Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe (3000), Argentina
LUIS M. GUGLIOTTA*
Affiliation:
INTEC (Universidad Nacional del Litoral and CONICET), Santa Fe (3000), Argentina
*
*Corresponding author: INTEC, Güemes 3450, Santa Fe, Argentina. E-mail: [email protected]
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Summary

The aim of this work was to obtain a reagent based on latex particles for ruling out acute toxoplasmosis in pregnant women by immunoagglutination (IA). Latex–protein complexes (LPC) were previously synthesized coupling the recombinant protein of Toxoplasma gondii P22Ag and the homogenate of the parasite to latex particles with different size, chemical functionality and charge density. LPC were tested in IA assays against a panel of 72 pregnant women serum samples. Results were analysed through receiver operating characteristic curves, determining area under the curve (AUC), sensitivity, specificity positive and negative predictive values (PPV and NPV, respectively). It was observed that the antigenicity of proteins was not affected during sensitization by either physical adsorption or covalent coupling. The best results in the sense of maximizing discrimination of low avidity sera from chronic ones were observed for the IA test based on latex particles with carboxyl functionality and the recombinant P22Ag, obtaining an AUC of 0·94, a sensitivity of 100% and a NPV of 100%. In this way, the proposed test could be useful for the toxoplasmosis diagnosis in pregnant women, with the advantages of being cheap, rapid and easy to be implemented.

Keywords

Toxoplasma gondiiacute toxoplasmosislatex–protein complexesacute-phase recombinant antigenimmunoagglutination
Type
Research Article
Information
Parasitology , Volume 144 , Issue 8 , July 2017 , pp. 1073 - 1078
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Copyright
Copyright © Cambridge University Press 2017 

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