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Development of Acanthocheilonema reconditum (Spirurida, Onchocercidae) in the cat flea Ctenocephalides felis (Siphonaptera, Pulicidae)

Published online by Cambridge University Press:  28 July 2014

ETTORE NAPOLI ,
EMANUELE BRIANTI ,
LUIGI FALSONE ,
GABRIELLA GAGLIO ,
SABRINA FOIT ,
FRANCESCA ABRAMO ,
GIADA ANNOSCIA ,
FILIPE DANTAS-TORRES ,
SALVATORE GIANNETTO  and
DOMENICO OTRANTO
ETTORE NAPOLI
Affiliation:
Dipartimento di Scienze Veterinarie, Università degli Studi di Messina, Messina, Italy
EMANUELE BRIANTI*
Affiliation:
Dipartimento di Scienze Veterinarie, Università degli Studi di Messina, Messina, Italy
LUIGI FALSONE
Affiliation:
Dipartimento di Scienze Veterinarie, Università degli Studi di Messina, Messina, Italy
GABRIELLA GAGLIO
Affiliation:
Dipartimento di Scienze Veterinarie, Università degli Studi di Messina, Messina, Italy
SABRINA FOIT
Affiliation:
Bayer Animal Health GmbH (BAH), Monheim, Germany
FRANCESCA ABRAMO
Affiliation:
Dipartimento di Patologia Animale, Università di Pisa, Pisa, Italy
GIADA ANNOSCIA
Affiliation:
Dipartimento di Medicina Veterinaria, Università di Bari, Valenzano, Bari, Italy
FILIPE DANTAS-TORRES
Affiliation:
Dipartimento di Medicina Veterinaria, Università di Bari, Valenzano, Bari, Italy Department of Immunology, Aggeu Magalhães Research Centre, Oswaldo Cruz Foundation, Recife, Pernambuco 50670420, Brazil
SALVATORE GIANNETTO
Affiliation:
Dipartimento di Scienze Veterinarie, Università degli Studi di Messina, Messina, Italy
DOMENICO OTRANTO
Affiliation:
Dipartimento di Medicina Veterinaria, Università di Bari, Valenzano, Bari, Italy
*
* Corresponding author: Dipartimento di Scienze Veterinarie, Università degli Studi di Messina, Polo Universitario dell'Annunziata, 98168, Messina, Italy. E-mail: [email protected]
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Summary

To investigate larval development of Acanthocheilonema reconditum in the cat flea Ctenocephalides felis, fleas were fed through an artificial feeding system with dog blood containing different concentrations of microfilariae (i.e. low, group L = 250; medium, group M = 500; high, group H = 1500 microfilariae per mL) or no microfilariae (group C). Fleas were sampled at 12 different time-points throughout the study period (D1–D28) and A. reconditum was detected by dissection, PCR and histology. Of 2105 fleas fed with infected dog blood, 891 (38·7%) died during the study before being sampled whilst the remaining (n = 1214) were examined for A. reconditum. Upon dissection, first-stage larvae (L1) were identified after 2 days post infection (D2), second-stage (L2) at D13 and infective third-stage larvae (L3) at D15. Eighteen (30%) of 60 pools of fleas molecularly examined tested positive. Histologically, L2 were detected at D13 in the sub-cuticle region embedded in the back muscle of one female flea. This study provides original data on larval development of A. reconditum in C. felis and reports on the usefulness of the artificial feeding system.

Keywords

Acanthocheilonema reconditumCtenocephalides felisbiologyfleaartificial feeding systemdog filariosis
Type
Research Article
Information
Parasitology , Volume 141 , Issue 13 , November 2014 , pp. 1718 - 1725
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Copyright
Copyright © Cambridge University Press 2014 

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