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Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced onto a ranch in Kilifi, Kenya

Published online by Cambridge University Press:  06 April 2009

R. A. Masake ,
V. M. Nantulya ,
A. J. Musoke ,
S. K. Moloo  and
K. Nguli
R. A. Masake
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
V. M. Nantulya
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
A. J. Musoke
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
S. K. Moloo
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
K. Nguli
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, KE
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Summary

A herd of 20 cattle was introduced on a ranch in Kilifi, Coast Province of Kenya, where they were in contact with Glossina austeni for 6 months. In total, 65 trypanosome isolates were made from these animals. Examination of the isolates revealed that 61 were Trypanosoma congolense and 4 were T. theileri. Out of the 61 T. congolense isolates, 55 were successfully passaged and cloned in mice to provide trypanosome populations for further analyses. The stocks and their clones were inoculated into goats on which teneral G. morsitans centralis were later fed in order to provide metacyclics for use in serodeme analysis. Identification of serodemes was carried out by indirect immunofluorescence and neutralization using antimetacyclic hyperimmune sera prepared in mice against metacyclics of cloned trypanosome populations. So far 4 serodemes have been identified in 8 stocks and 7 clones. Each of the 9 stocks contained a mixture of at least 2 of the 4 serodemes identified. Furthermore, stocks isolated sequentially from individual animals contained the same serodemes despite repeated treatment with a curative dose (6 mg/kg body weight) of Berenil between isolations. From the latter finding, it can be inferred that the 4 serodemes were present on the ranch throughout the study period.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 349 - 357
Copyright
Copyright © Cambridge University Press 1987

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