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Characterization of the relationships in the pinewood nematode species complex (PWNSC) (Bursaphelenchus spp.) using a heterologous unc-22 DNA probe from Caenorhabditis elegans

Published online by Cambridge University Press:  06 April 2009

P. Abad ,
S. Tares ,
N. Brugier  and
G. De Guiran
P. Abad
Affiliation:
I.N.R.A. Station de Nématologie et de Génétique Moléculaire des Invertébrés, 123, Bld F. Meilland, 06606 Antibes Cedex
S. Tares
Affiliation:
I.N.R.A. Station de Nématologie et de Génétique Moléculaire des Invertébrés, 123, Bld F. Meilland, 06606 Antibes Cedex
N. Brugier
Affiliation:
I.N.R.A. Station de Nématologie et de Génétique Moléculaire des Invertébrés, 123, Bld F. Meilland, 06606 Antibes Cedex
G. De Guiran
Affiliation:
I.N.R.A. Station de Nématologie et de Génétique Moléculaire des Invertébrés, 123, Bld F. Meilland, 06606 Antibes Cedex
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Summary

Pine wilt is the most serious disease of native pines in Japan and potentially the most important nematode disease of conifers in the world. The pinewood nematode Bursaphelenchus xylophilus was found to be the causal agent. Difficulties arose with respect to the precise identity of some isolates of B. xylophilus and of similar species B. mucronatus and B. fraudulentus. Restriction enzyme analyses of repetitive DNA revealed bands specific for the species B. xylophilus, B. mucronatus and B. fraudulentus. Hybridization patterns obtained with unc-22 gene of C. elegans, clearly identified B. xylophilus, B. mucronatus and B. fraudulentus as well as the different geographic isolates of these species. Furthermore, it is possible to define the phylogenetic relationships between the different populations constituting the ‘pine wood nematode’ complex.

Keywords

pine wood nematodes species complexBursaphelenchusDNAhybridization patterns
Type
Research Article
Information
Parasitology , Volume 102 , Issue 2 , April 1991 , pp. 303 - 308
Copyright
Copyright © Cambridge University Press 1991

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