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Characterization and immunolocalization of an oocyst wall antigen of Cryptosporidium parvum (Protozoa: Apicomplexa)

Published online by Cambridge University Press:  06 April 2009

A. Bonnin ,
J. F. Dubremetz  and
P. Camerlynck
A. Bonnin
Affiliation:
Laboratoire de Parasitologie-Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
J. F. Dubremetz
Affiliation:
INSERM U42, Domaine du Certia, 59650 Villeneuve d'Ascq, France
P. Camerlynck
Affiliation:
Laboratoire de Parasitologie-Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France
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Abstract

A monoclonal antibody (OW-IGO) raised against purified excysted oocysts of Cryptosporidium parvum reacted in an immunofluorescence assay with the oocyst wall. The corresponding antigen was localized by immunoelectron microscopy in fibrillous material present in the parasitophorous vacuole of developing macrogametes and in the wall of both single and double layered sporulating oocysts. Gold particles were also detected over electron-lucent vesicles of the macrogametes by immunoelectron microscopy. On Western blotting of C. parvum oocyst extracts, major bands at 250 and 40 kDa and several minor components were recognized by Mab OW-IGO. Almost complete abolition of Western blot reactivity occurred after periodate oxidation of oocyst antigen, suggesting that monoclonal antibody OW-IGO reacts with a carbohydrate epitope. Taken together, our results suggest that a fibrillous glycoproteinic material is released in the parasitophorous vacuole from electron-lucent vesicles during gametogenesis, and later condensed in the oocyst wall.

Keywords

Cryptosporidium parvumoocyst wallantigenglycoproteinmonoclonal antibody
Type
Research Article
Information
Parasitology , Volume 103 , Issue 2 , October 1991 , pp. 171 - 177
Copyright
Copyright © Cambridge University Press 1991

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