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Association between circulating antigen and parasite load in a model filarial system, Acanthocheilonema viteae in jirds

Published online by Cambridge University Press:  06 April 2009

W. Harnett ,
M. J. Worms ,
M. Grainger ,
S. D. M. Pyke  and
R. M. E. Parkhouse
W. Harnett
Affiliation:
Divisions of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
M. J. Worms
Affiliation:
Divisions of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
M. Grainger
Affiliation:
Divisions of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
S. D. M. Pyke
Affiliation:
Mathematical Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
R. M. E. Parkhouse
Affiliation:
Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
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Summary

Jirds (Meriones libycus) were infected with various numbers of Acanthocheilonema viteae L3 stage parasites. During the course of the ensuing 16 weeks, blood samples were collected at 2 weekly intervals and the amount of the major parasite excretory–secretory product (E–S 62) and antibodies directed against it measured. After 16 weeks, animals were sacrificed and the size of the mature worm burden established. In spite of interaction between E–S 62 and host antibody, a statistically significant relationship was found to exist between the amount of E–S 62 present in the bloodstream and the size of the parasite load. It is suggested that the detectable antigen level is more influenced by the size of the worm burden than the presence of antibody and that antibody is only likely to affect adversely antigen measurement in situations where the amount released is relatively low. Examples of this are early in infection and in low-level infections. These ideas are discussed in relation to the development and assessment of serological assays which attempt to predict parasite burden in human filarial infections.

Keywords

Acanthocheilonema viteaeantigencirculating antigenexcretory–secretory antigendiagnosisfilaria
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 435 - 444
Copyright
Copyright © Cambridge University Press 1990

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