Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-15T07:24:21.999Z Has data issue: false hasContentIssue false
  • English
  • Français

Assignment of Vairimorpha leptinotarsae comb. nov. on the basis of molecular characterization of Nosema leptinotarsae Lipa, 1968 (Microsporidia: Nosematidae)

Published online by Cambridge University Press:  27 April 2020

Çağrı Bekircan Open the ORCID record for Çağrı Bekircan [Opens in a new window]
Çağrı Bekircan*
Affiliation:
Department of Veterinary, Sarayönü Vocational School, Selçuk University, 42430, Konya, Turkey
*
Author for correspondence: Çağrı Bekircan, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Nosema leptinotarsae Lipa, 1968 is a microsporidian pathogen of the Colorado potato beetle, Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae). To determine the phylogenetic status of N. leptinotarsae, the 16S SSU rRNA gene was sequenced (GenBank Accession No. MN841279) and compared phylogenetically against 21 microsporidian 16S SSU rRNA sequences using neighbour-joining and maximum-parsimony methods. The per cent identities of the N. leptinotarsae and other members of the Nosema–Vairimorpha clade ranged from 78.1 to 98.5%. Pairwise phylogenetic distances between the N. leptinotarsae and other species ranged from 0.009 to 0.320. Phylogenetic analysis shows clearly that N. leptinotarsae is a member of the Vairimorpha clade rather than the Nosema clade. The sequence divergence and morphological traits separated the N. leptinotarsae from other species in the Vairimorpha complex. As a result, a new assignment of Vairimorpha leptinotarsae comb. nov. has been implemented for N. leptinotarsae according to the phylogenetical positioning in the present study.

Keywords

ChrysomelidaeLeptinotarsa decemlineataMicrosporidiaNosema leptinotarsaepotatoVairimorpha
Type
Research Article
Information
Parasitology , Volume 147 , Issue 9 , August 2020 , pp. 1019 - 1025
Check for updates
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baker, MD, Vossbrinck, CR, Maddox, JV and Undeen, AH (1994) Phylogenetic relationships among Vairimorpha and Nosema species (Microspora) based on ribosomal RNA sequence data. Journal of Invertebrate Pathology 64, 100–106.CrossRefGoogle ScholarPubMed
Baker, MD, Vossbrinck, CR, Didier, ES, Maddox, JV and Shadduck, JA (1995) Small subunit ribosomal DNA phylogeny of various Microsporidia with emphasis on AIDS related forms. Journal of Eukaryotic Microbiology 42, 564–570.CrossRefGoogle ScholarPubMed
Baker, MD, Vossbrinck, CR, Becnel, JJ and Maddox, JV (1997) Phylogenetic position of Amblyospora Hazard and Oldacre (Microspora, Amblyosporidae) based on small subunit rRNA data and its implication for the evolution of the Microsporidia. Journal of Eukaryotic Microbiology 44, 220–225.CrossRefGoogle ScholarPubMed
Balbiani, G (1882) Surles microsporidies ou psorospermies des articules. Comptes Rendus de l'Academie des Sciences 95, 1168–1171.Google Scholar
Becnel, JJ, Jeyaprakash, A, Hoy, MA and Shapiro, A (2002) Morphological and molecular characterization of a new microsporidian species from the predatory mite Metaseiulus occidentalis (Nesbitt) (Acari, Phytoseiidae). Journal Of Invertebrate Pathology 79, 163–172.CrossRefGoogle Scholar
Bekircan, Ç, Cüce, M, Baki, H and Tosun, O (2017a) Aranciocystis muskarensis n. gen., n. sp., a neogregarine pathogen of the Anisoplia segetum Herbst (Coleoptera: Scarabaeidae). Journal of Invertebrate Pathology 144, 58–64.CrossRefGoogle Scholar
Bekircan, Ç, Bülbül, B, Güler, Hİ and Becnel, JJ (2017b) Description and phylogeny of a new microsporidium from the elm leaf beetle Xanthogaleruca luteola Muller 1766 (Coleoptera Chrysomelidae). Parasitology Research 116, 773–780.CrossRefGoogle Scholar
Canning, EU and Hazard, EI (1982) Genus Pleistophora Gurley, 1893: an assemblage of at least three genera. The Journal of Protozoology 29, 39–49.CrossRefGoogle Scholar
Canning, EU andVávra, J (2000) Phylum microsporida. In Lee, JJ, Leedale, GF and Bradbury, P (eds), The Illustrated Guide to the Protozoa. Lawrence: Allen Press Inc., pp. 39–126.Google Scholar
Ertürk, Ö, Yaman, M and Aslan, İ (2008) Effects of four Bacillus spp. of soil origin on the Colorado potato beetle Leptinotarsa decemlineata (Say). Entomological Research 38, 135–138.CrossRefGoogle Scholar
Hare, JD (1980) Impact of defoliation by the Colorado potato beetle on potato yields. Journal of Economic Entomology 73, 369–373.CrossRefGoogle Scholar
Hare, JD (1990) Ecology and management of the Colorado potato beetle. Annual Review of Entomology 35, 81–100.CrossRefGoogle Scholar
Hostounský, Z (1984) Production of microsporidia pathogenic to the Colorado potato beetle (Leptinotarsa decemlineata) in alternate hosts. Journal of Invertebrate Pathology 44, 166–171.CrossRefGoogle Scholar
Huang, WF, Tsai, SJ, Lo, CF, Soichi, Y and Wang, CH (2004) The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis. Fungal Genetics and Biology 41, 473–481.CrossRefGoogle ScholarPubMed
Issi, IV (1986) Microsporidia as a phylum of parasitic protozoa. Protozoology 10, 1–136.Google Scholar
Kramer, JP (1965) Nosema necatrix sp. n. and Thelohania diazoma sp. n., microsporidians from the armyworm Pseudaletia unipuncta (Haworth). Journal of Invertebrate Pathology 7, 117–121.CrossRefGoogle Scholar
Kudo, R (1924) A biologic and taxonomic study of the Microsporidia. Illinois Biological Monographs 9, 1–268.Google Scholar
Labbe, A, (1899) Sporozoa. In Schulze, FE and Butschli, O (eds), Das Tierreich. Eine Zusammenstellung und Kennzeichnung der Rezenten Tierformen. Berlin: W. de Gruyter, 5, xx + 180 pp.Google Scholar
Larsson, R (1986) Ultrastructure, function and classification of microsporidia. Progress in Protistology 1, 325–390.Google Scholar
Larsson, JIR and Voronin, VN (2000) Light and electron microscopic study of Agglomerata volgensae n. sp. (Microspora: Dubosqiidae), a new microsporidian parasite of Daphnia magna (Crustacea: Daphniidae). European Journal of Protistology 36, 89–99.CrossRefGoogle Scholar
Lewis, LC (2007) Protozoan control of pests. In Pimentel, D (ed.), Encyclopedia of Pest Management. New York: CRC Press Inc, pp. 673–680.Google Scholar
Lipa, JJ (1968) Nosema leptinotarsae sp. n., a microsporidian parasite of the Colorado potato beetle, Leptinotarsa decemlineata (Say). Journal of Invertebrate Pathology 10, 111–115.CrossRefGoogle Scholar
Luo, B, Liu, H, Pan, G, Li, T, Li, Z, Dang, X, Liu, T and Zhou, Z (2014) Morphological and molecular studies of Vairimorpha necatrix BM, a new strain of the microsporidium V. necatrix (Microsporidia, burenellidae) recorded in the silkworm, Bombyx mori. Experimental Parasitology 143, 74–82.CrossRefGoogle ScholarPubMed
Maddox, JV and Sprenkel, RK (1978) Some enigmatic microsporidia of the genus Nosema. Miscellaneous Publications of the Entomological Society of America 11, 65–84.Google Scholar
Malone, LA and Wigley, PJ (1981) The morphology and development of Nosema carpocapsae, a microsporidian pathogen of the codling moth, Cydia pomonella (Lepidoptera: Tortricidae) in New Zealand. Journal of Invertebrate Pathology 38, 315–329.CrossRefGoogle Scholar
Martin, PAW (2004) A freeze-dried diet to test pathogens of Colorado potato beetle. Biological Control 29, 109–114.CrossRefGoogle Scholar
Mennan, S and Ertürk, Ö (2006) First record of parasitism of a Mermithidae (Nematoda) on Leptinotarsa decemlineata (Col: Chrysomelidae) in Turkey. Pakistan Journal of Nematology 24, 39–43.Google Scholar
Mitchell, MJ and Cali, A (1993) Ultrastructural study of the development of Vairimorpha necatrix (Kramer, 1965) (Protozoa, Microsporida) in larvae of the corn earworm, Heliothis zea (Boddie) (Lepidoptera, Noctuidae) with emphasis on sporogony. Journal of Eukaryotic Microbiology 40, 701–710.CrossRefGoogle Scholar
Muratoğlu, H, Demirbağ, Z and Sezen, K (2011) The first investigation of the diversity of bacteria associated with Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Biologia 66, 288–293.CrossRefGoogle Scholar
Nägeli, C (1857) Überdie neue Krankheit der Seidenraupe und verwandte Organismen. Botanische Zeitung 15, 60–761.Google Scholar
O'Neil, RJ, Cañas, LA and Obrycki, JJ (2005) Foreign exploration for natural enemies of the Colorado potato beetle in Central and South America. Biological Control 33, 1–8.CrossRefGoogle Scholar
Ovcharenko, M, Swiatek, P, Ironside, J and Skalski, T (2013) Orthosomella lipae sp. n. (Microsporidia) a parasite of the weevil, Liophloeus lentus Germar, 1824 (Coleoptera: Curculionidae). Journal of Invertebrate Pathology 112, 33–40.CrossRefGoogle Scholar
Pilley, BM (1976) A new genus, Vairimorpha (Protozoa: Microsporida), for Nosema necatrix Kramer 1965: pathogenicity and life cycle in Spodoptera exempta (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology 28, 177–183.CrossRefGoogle Scholar
Reynolds, ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17, 208–212.CrossRefGoogle ScholarPubMed
Schroder, RF and Athanas, MM (1989) Potential for the biological control of Leptinotarsa decemlineata (Col.: Chrysomelidae) by the egg parasite, Edovum puttleri (Hym.: Eulophidae) in Maryland, 1981–84. Entomophaga 34, 135–141.CrossRefGoogle Scholar
Sidor, Ć (1974) Survey of some results obtained in investigations of diseases of Colorado potato beetle Leptinotarsa decemlineata Say. Agronomski Glusnik, br 9–12, 537–546.Google Scholar
Sokolova, YY, Senderskiy, IV and Tokarev, YS (2016) Microsporidia Alfvenia sibirica sp. n. and Agglomerata cladocera (Pfeiffer) 1895, from Siberian microcrustaceans and phylogenetic relationships within the ‘Aquatic outgroup’ lineage of fresh water microsporidia. Journal of Invertebrate Pathology 136, 81–91.CrossRefGoogle Scholar
Sprague, V, Becnel, JJ and Hazard, EI (1992) Taxonomy of phylum microspora. Critical Reviews in Microbiology 18, 285–395.CrossRefGoogle ScholarPubMed
Spurr, AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. Clinical Microbiology Research 3, 197–218.Google Scholar
Tokarev, YS, Huang, W-F, Solter, LF, Malysh, JM, Becnel, JJ and Vossbrinck, CR (2020) A formal redefinition of the genera Nosema and Vairimorpha (Microsporidia: Nosematidae) and reassignment of species based on molecular phylogenetics. Journal of Invertebrate Pathology 169, 107279.CrossRefGoogle ScholarPubMed
Undeen, AH andVávra, J (1997) Research methods for entomopathogenic protozoa. In Lacey, L (ed.), Manual of Techniques in Insect Pathology. Biological Techniques Series. London: Academic Press, pp. 117–151.CrossRefGoogle Scholar
Vavra, J and Sprague, V (1976) Biology of the microsporidia. In Bulla, LA and Cheng, TC (eds), Comparative Pathobiology Vol: 1. New York: Plenum Press, p. 371.Google Scholar
Voronin, VN (2001) On a macrotaxonomy of the phylum Microsporidia. Parazitologiya 35, 35–44, (in Russian).Google Scholar
Weiser, J (1977) Contribution to the classification of Microsporidia. Vestnik Ceskoslovenske Spolecnosti Zoologicke 41, 308–320.Google Scholar
Weiss, LM and Becnel, JJ (2014) Microsporidia: Pathogens of Opportunity. Oxford: Wiley-Blackwell.CrossRefGoogle Scholar
Yaman, M, Özcan, N, Radek, R, Linde, A and Lipa, J (2011) Ultrastructure, characteristic features and occurrence of Nosema leptinotarsae Lipa, 1968, a microsporidian pathogen of Leptinotarsa decemlineata (Coleoptera, Chrysomelidae). Acta Protozoologica 56, 1–7.Google Scholar