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Assessment of monoclonal antibodies to Echinococcus granulosus Antigen 5 and Antigen B for detection of human hydatid circulating antigens

Published online by Cambridge University Press:  06 April 2009

D. Liu ,
M. D. Rickard  and
M. W. Lightowlers
D. Liu*
Affiliation:
University of Melbourne, Veterinary Clinical Centre, Princes Highway, Werribee, Victoria 3030, Australia
M. D. Rickard
Affiliation:
C.S.I.R.O. Division of Animal Health, Private Bag No. 1, Parkville, Victoria 3052, Australia
M. W. Lightowlers
Affiliation:
University of Melbourne, Veterinary Clinical Centre, Princes Highway, Werribee, Victoria 3030, Australia
*
*D. Liu, Department of Food and Agriculture, Pastoral and Veterinary Institute, Ballarat Road, Hamilton, Victoria 3300, Australia
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Summary

Four monoclonal antibodies (MAb) to Echinococcus granulosus Antigen 5 (Ag5) and Antigen B (AgB) were assessed in an enzyme-linked immunosorbent assay (ELISA) for detection of circulating antigens (CAg) in sera of human patients with E. granulosus infection. Around 5·5–8% of 200 sera from 42 surgically proven hydatid patients contained detectable CAg by individual MAb. The combined detection rate for CAg, using four MAb, was 19% (38/200). Although hydatid CAg was detected by MAb in at least one serum sample from 21 of 42 patients, some patients remained negative in the assay regardless of the time when serum samples were taken (pre- or post-operatively), or of the continuing presence of hydatid cysts, their location or fertility. In addition, it was observed that the binding capacity of MAb for sheep hydatid cyst fluid antigen (SHCF) was somewhat reduced in the presence of normal human serum. The CAg detection assay would only be useful for assessment of hydatid infection status in patients with detectable CAg in serum samples.

Keywords

Echinococcus granulosusAntigen 5Antigen Bmonoclonal antibodycirculating antigenhydatidosis
Type
Research Article
Information
Parasitology , Volume 106 , Issue 1 , January 1993 , pp. 75 - 81
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Bekhti, A., Schaaps, J. P., Capron, M., Dessaint, J. P., Santoro, F. & Capron, A. (1977). Treatment of hepatic hydatid disease with mebendazole: preliminary results in four cases. British Medical Journal 2, 1047–51.CrossRefGoogle ScholarPubMed
Chandler, H. M., Cox, J. C., Healey, K., Macgregor, A., Premier, R. R. & Hurrell, J. G. R. (1982). An Investigation of use of urease-antibody conjugates in enzyme immunoassays. Journal of Immunological Methods 53, 187–94.CrossRefGoogle ScholarPubMed
Chemtai, A. K., Bowry, T. R. & Ahmad, Z. (1981). Evaluation of five immunodiagnostic techniques in echinococcosis patients. Bulletin of the World Health Organization 59, 767–72.Google ScholarPubMed
Craig, P. S. (1986). Detection of specific circulating antigen, immune complexes and antibodies in human hydatidosis from Turkana (Kenya) and Great Britain, by enzyme-immunoassay. Parasite Immunology 8, 171–88.CrossRefGoogle ScholarPubMed
Craig, P. S. & Nelson, G. S. (1984). The detection of circulating antigen in human hydatid disease. Annals of Tropical Medicine and Parasitology 78, 219–27.CrossRefGoogle ScholarPubMed
Farag, H., Bout, D. & Capron, A. (1975). Specific immunodiagnosis of human hydatidosis by the enzyme-linked immunosorbent assay (ELISA). Biomedicine 23, 276–8.Google Scholar
Feldmeier, H., Nogueira-Queiroz, J. A., Peixoto-Queiroz, M. A., Doehring, E., Dessaint, J. P., Alencar, J. E. De, Dafalla, A. A. & Capron, A. (1986). Detection and quantification of circulating antigen in schistosomiasis by monoclonal antibody. II. The quantification of circulating antigens in human schistosomiasis mansoni and haematobium: relationship to intensity of infection and disease status. Clinical and Experimental Immunology 65, 232–43.Google ScholarPubMed
Gottstein, B. (1984). An immunoassay for the detection of circulating antigens in human echinococcosis. American Journal of Tropical Medicine and Hygiene 33, 1185–91.CrossRefGoogle ScholarPubMed
Kato, K., Umeda, Y., Suzuki, F. & Kosaka, A. (1979). Interference in a solid-phase enzyme immunoassay system by serum factors. Journal of Applied Biochemistry 1, 479–88.Google Scholar
Lew, M. A., Siber, G. R., Donahue, D. M. & Maiorca, F. (1982). Enhanced detection with an enzyme-linked immunosorbent assay of Candida mannan in antibody-containing serum after heat extraction. Journal of Infectious Diseases 145, 45–56.CrossRefGoogle ScholarPubMed
Lightowlers, M. W., Liu, D., Haralambous, A. & Rickard, M. D. (1989). Subunit composition and specificity of the major cyst fluid antigens of Echinococcus granulosus. Molecular and Biochemical Parasitology 37, 171–82.CrossRefGoogle ScholarPubMed
Liu, D., Lightowlers, M. W. & Rickard, M. D. (1992 a). Evaluation of a monoclonal antibody based competition ELISA for diagnosis of human hydatidosis. Parasitology 104, 357–61.CrossRefGoogle ScholarPubMed
Liu, D., Rickard, M. D. & Lightowlers, M. W. (1992 b). Further characterisation of monoclonal antibodies to Echinococcus granulosus Antigen 5 and Antigen B. International Journal for Parasitology 22, 391–4.CrossRefGoogle ScholarPubMed
Pini, C., Pastore, R. & Valesini, G. (1983). Circulating immune complexes in sera of patients infected with Echinococcus granulosus. Clinical and Experimental Immunology 51, 572–8.Google ScholarPubMed
Richard-Lenoble, D., Smith, M. D., Loisy, M. & Verroust, P. J. (1978). Human hydatidosis: evaluation of three serodiagnostic methods, the principal subclass of specific immunoglobulin and the detection of circulating immune complexes. Annals of Tropical Medicine and Parasitology 72, 553–60.CrossRefGoogle ScholarPubMed
Rickard, M. D. (1984). Serological diagnosis and post-operative surveillance of human hydatid disease. I. Latex agglutination and immunoelectrophoresis using crude cyst fluid antigen. Pathology 16, 207–10.CrossRefGoogle ScholarPubMed
Rickard, M. D., Honey, R. D., Brumley, J. L. & Mitchell, G. F. (1984). Serological diagnosis and post-operative surveillance of human hydatid disease. II. The enzyme-linked immunosorbent assay (ELISA) using various antigens. Pathology 16, 211–15.CrossRefGoogle ScholarPubMed
Robertson, B. D., Burkot, T. R., Gillespie, S. H., Kennedy, M. W., Wambai, Z. & Maizels, M. (1988). Detection of circulating parasite antigen and specific antibody in Toxocara canis infections. Clinical and Experimental Immunology 74, 236–41.Google ScholarPubMed
Shantz, P. M., Shanks, D. & Wilson, M. (1980). Serologic cross-reactions with sera from patients with echinococcosis and cysticercosis. American Journal of Tropical Medicine and Hygiene 29, 609–12.CrossRefGoogle Scholar
Speiser, F. (1980). Application of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of filariasis and echinococcosis. Tropenmedizin und Parasitologie 31, 459–66.Google ScholarPubMed
Weil, G. J., Malane, M. S., Powers, K. G. & Blair, L. S. (1985). Monoclonal antibodies to parasite antigens found in the serum of Dirofilaria immitis-infected dogs. Journal of Immunology 134, 1185–91.CrossRefGoogle ScholarPubMed