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Analysis and comparison of immune reactivity in guinea-pigs immunized with equivalent numbers of normal or radiation-attenuated cercariae of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

M. V. Rogers  and
Diane J. McLaren
M. V. Rogers
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Diane J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

Guinea-pigs immunized with equivalent numbers of normal or radiation-attenuated cercariae of Schistosomamansoni are known todevelop close to complete resistance to reinfection at weeks 12 and 4·5 respectively. We here analyse and compare the immune responses induced by the two populations of cercariae. Both radiation-attenuated and normal parasites of S. mansoni elicited an extensive germinal centre response in guinea-pigs by week 4·5 post-immunization. The anti-parasite antibody titre and cytotoxic activity of serum from 4·5-week-vaccinated, or 4·5-week-infected guinea-pigs were approximately equal, but sera from 12-week-infected individuals had high titres of anti-parasite antibody, which promoted significant larvicidal activity in vitro. In all cases, larvicidal activity was mediated by the IgG2 fraction of the immune serum. Lymphocyte transformation tests conducted on splenic lymphocytes from 4–5-week vaccinated guinea-pigs revealed maximal stimulation against cercarial, 2-week and 3-week worm antigens, whereas spleen cells from 4–5-week-infected guinea-pigs were maximally stimulated by cercarial and 6-week worm antigens. The splenic lymphocyte responses of 12-week infected animals were dramatic against antigens prepared from all life-stages of the parasite.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 1 , August 1987 , pp. 43 - 59
Copyright
Copyright © Cambridge University Press 1987

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