Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-27T23:55:04.041Z Has data issue: false hasContentIssue false
  • English
  • Français

An ELISA for the detection of pentastomid infections in the rat

Published online by Cambridge University Press:  06 April 2009

D. A. C. Jones  and
J. Riley
D. A. C. Jones
Affiliation:
Department of Biological Sciences, The University of Dundee, Dundee DD1 4HN, Scotland
J. Riley
Affiliation:
Department of Biological Sciences, The University of Dundee, Dundee DD1 4HN, Scotland
Get access Rights & Permissions [Opens in a new window]

Extract

A specific enzyme-linked immunosorbent assay, based on a 48 kDa metallo-proteinase isolated from the frontal glands of a porocephalid pentastomid (Porocephalus crotali) in rat intermediate hosts, is described. SDS–PAGE analysis of whole nymphal homogenate, followed by Western blotting and probing with immune rat serum, revealed that only 2 protein bands label consistently; these are the 48 kDa metallo-proteinase and a 150 kDa metallo-proteinase from the body fluid, but only the former labels strongly. Furthermore this response is detected earlier and persists much longer. These characteristics uniquely qualify the 48 kDa metallo-proteinase as a diagnostic antigen. We review the literature concerning the related porocephalid Linguatula serrata from the nasal cavities of domestic dogs, which can zoonotically infect man principally through the ingestion of eggs. We conclude that the high prevalences in dogs in certain areas, most notably the Middle East, must inevitably signal significant human infections. Our ELISA can be readily adapted for the serodiagnosis of linguatulosis and this should establish whether our prediction is true.

Keywords

pentastomidserodiagnosiszoonosisELISA
Type
Research Article
Information
Parasitology , Volume 103 , Issue 3 , December 1991 , pp. 331 - 337
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abele, L. G., Kim, W. & Felgenhauer, B. E. (1989). Molecular evidence for the inclusion of the phylum Pentastomida in the Crustacea. Molecular and Biochemical Evolution 6, 685–91.Google Scholar
Ambrose, N. C. & Riley, J. (1988 a). Light microscope observations of granulomatous reactions against developing Porocephalus crotali (Pentastomida: Porocephalida) in mouse and rat. Parasitology 97, 1–15.CrossRefGoogle ScholarPubMed
Ambrose, N. C. & Riley, J. (1988 b). Fine structural aspects of secretory processes in a pentastomid arthropod parasite in its mouse and rattlesnake hosts. Tissue and Cell 20, 381–404.CrossRefGoogle Scholar
Ambrose, N. C. & Riley, J. (1988 c). Studies on the host parasite interface during the development of a pentastomid arthropod parasite in rodent intermediate hosts, with observations on protective surface membranes. Tissue and Cell 20, 721–44.CrossRefGoogle ScholarPubMed
Ambrose, N. C. & Riley, J. (1989). Further evidence for the protective role of sub-parietal cell membranous secretory product on the cuticle of a pentastomid arthropod parasite developing in its rodent intermediate host. Tissue and Cell 21, 699–722.CrossRefGoogle ScholarPubMed
Amy, D., Ranque, P., Discamps, G., Rougemont, A. & Quilici, M. (1974). La pentastomose Humaine. Médecine Tropicale 34, 273–8.Google Scholar
Ardran, G. M. (1948). Armillifer armiallatus. A note on three cases of calcification of the cysts in man. British Journal of Radiology, New Series 21, 342–5.CrossRefGoogle Scholar
Baird, J. K., Kassebaum, L. J. & Ludwig, G. K. (1988). Hepatic granuloma in a man from North America caused by a nymph of Linguatula serrata. Pathology 20, 198–9.CrossRefGoogle Scholar
Dorchies, P., Ducos, D. E, Lahitte, J., Pangui, L. J. & Alzieu, J. P. (1988). Recherche de Fasciola hepatica, Dicrocoelium lanceolatum et Linguatula denticulata dans les foies de bovins saisis à l'abattoire de Pamiers. Revue de Médecine Vétérinaire 139, 307–9.Google Scholar
Doucet, J. (1965). Contribution à l'étude anatomique, histologique et histochimique des pentastomes (Pentastomida). Mémoires de l'office de la Recherche Scientifique et Technique Outre-Mer, Paris 14, 1–50.Google Scholar
Ehrenford, F. A. & Newberne, J. W. (1981). An aid to the clinical diagnosis of tongue worms (Linguatula serrata) in dogs. Laboratory Animal Science 31, 74–6.Google Scholar
Esslinger, J. H. (1962). Development of Porocephalus crotali (Humboldt, 1808) (Pentastomida) in experimental intermediate hosts. Journal of Parasitology 48, 452–6.CrossRefGoogle Scholar
Fain, A. (1961). Les pentastomides de l'afrique centrale. Annales du Musée Royale de l'afrique Centrale, Série 8, 92, 1–115.Google Scholar
Haralabidis, S. T., Papazachariadou, M. G., Koutinas, A. F. & Rallis, T. S. (1988). A survey on the prevalence of gastrointestinal parasites of dogs in the area of Thessaloniki, Greece. Journal of Helminthology 62, 45–9.CrossRefGoogle ScholarPubMed
Haugerud, R. E. (1989). Evolution of the pentastomids. Parasitology Today 5, 126–32.CrossRefGoogle ScholarPubMed
Herzog, U., Marty, P. & Zak, F. (1985). Pentastomiasis: case report of an acute abdominal emergency. Acta Tropica 42, 261–71.Google ScholarPubMed
Hobmaier, A. & Hobmaier, M. (1940). On the life cycle of Linguatula rhinaria. American Journal of Tropical Medicine 20, 199–210.Google Scholar
Hopps, H. C., Keegan, H. L., Price, D. L. & Self, J. T. (1971). Pentastomiasis. In Pathology of Protozoal and Helminthic Diseases (ed. Marcial-Roajas, R. A.), pp. 970–89. Baltimore: Williams and Wilkins.Google Scholar
Jones, D. A. C., Riley, J., Kerby, N. W. & Knox, D. P. (1991). Isolation and preliminary characterisation of a 48 kDa metalloproteinase from the excretory/secretory components of the frontal glands of Porocephalus pentastomids. Molecular and Biochemical Parasitology 46, 61–72.CrossRefGoogle ScholarPubMed
Kennedy, M. W. (1989). Genetic control of the immune repertoire in nematode infections. Parasitology Today 5, 316–24.CrossRefGoogle ScholarPubMed
Khalil, G. M. (1970). Incidence of Linguatula serrata infection in Cairo mongrel dogs. Journal of Parasitology 56, 485.CrossRefGoogle ScholarPubMed
Khalil, G. M. (1972). Linguatula serrata (Pentastomida) parasitizing humans and animals in Egypt, neighbouring countries and elsewhere: a review. Journal of the Egyptian Public Health Association 47, 363–9.Google Scholar
Khalil, G. M. (1973). Linguatula serrata from mongrel dogs in El-Dakhla Oasis (Egypt). Journal of Parasitology 59, 288.CrossRefGoogle ScholarPubMed
Khalil, G. M. & Schacher, J. F. (1965). Linguatula serrata in relation to halzoun and the marrara syndrome. American Journal of Tropical Medicine and Hygiene 14, 736–46.CrossRefGoogle ScholarPubMed
Kirchner, T. & Altmann, H. N. (1987). Parasitenlarven als Ursache umschriebener Leberherde. Morphologie und Differentialdiagnose. Pathologie 8, 31–6.Google Scholar
Lang, Y., Garzozi, H., Epstein, Z., Barkay, S., Gold, D. & Lengy, J. (1987). Intraocular pentastomiasis causing unilateral glaucoma. British Journal of Ophthalmology 71, 391–5.CrossRefGoogle ScholarPubMed
Lindner, R. R. (1965). Retrospective X-ray survey for porocephalosis. Journal of Tropical Medicine and Hygiene 68, 155–6.Google Scholar
Mehlhorn, H. (1988). Parasitology in Focus. New York: Springer-Verlag.CrossRefGoogle Scholar
Pandey, V. S., Dakkak, A. & Elmanoune, M. (1987). Parasites of stray dogs in the Rabat region, Morocco. Annals of Tropical Medicine and Parasitology 81, 53–5.CrossRefGoogle Scholar
Prathap, K., Lau, K. S. & Bolton, J. M. (1969). Pentastomiasis: a common finding at autopsy among Malaysian aborigines. American Journal of Tropical Medicine and Hygiene 18, 20–7.CrossRefGoogle ScholarPubMed
Riley, J. (1986). The biology of pentastomids. Advances in Parasitology 25, 45–128.CrossRefGoogle ScholarPubMed
Riley, J. & Banaja, A. A. (1975). Some ultrastructural observations on the integument of a pentastomid. Tissue and Cell 7, 33–50.CrossRefGoogle ScholarPubMed
Schacher, J. F., Saab, S., Germanos, R. & Boustany, N. (1969). The aetiology of halzoun in Lebanon: recovery of Linguatula serrata nymphs from two patients. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 854–8.CrossRefGoogle ScholarPubMed
Schaeffer, H. (1912). Ueber das Vorkommen von Porocephalus moniliformis in Kamerun. Archiv für Schiffs- und Tropenhygiene 16, 109–13.Google Scholar
Sieffert, H. (1910). Weitere Funde von Porocephalus moniliformis in Kamerun. Archiv für Schiffs- und Tropenhygiene 14, 506–14.Google Scholar
Sinclair, K. B. (1954). The incidence and life cycle of Linguatula serrata (Fröhlich 1789) in Great Britain. Journal of Comparative Pathology 64, 371–83.CrossRefGoogle ScholarPubMed
Smith, J. A., Oladiran, B., Lagundoye, S. B., Lawson, E. A. L. & Francis, T. I. (1975). Pentastomiasis and malignancy. Annals of Tropical Medicine and Parasitology 69, 503–12.CrossRefGoogle Scholar
Tasan, E. (1987). Elazig Kirsal yore kopeklerinde Linguatula serrata (Fröhlich, 1789) nin yayilisi. Doga Veterinerlik ve Hayvancilk 11, 86–9.Google Scholar
Van Wymeersch, H. & Wanson, M. (1954). Le porocéphalose nymphale et son image radiologique. Annales de la Société Belge de Médecine Tropicale 34, 517–22.Google Scholar