Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-19T19:29:32.123Z Has data issue: false hasContentIssue false
  • English
  • Français

An association between Schistosoma mansoni worms and an enzymatically-active protease/peptidase in mouse blood

Published online by Cambridge University Press:  22 January 2008

H. Y. DARANI  and
M. J. DOENHOFF
H. Y. DARANI*
Affiliation:
School of Biological Sciences, University of Bangor, Bangor, Gwynedd LL57 2UW, UK
M. J. DOENHOFF
Affiliation:
School of Biological Sciences, University of Bangor, Bangor, Gwynedd LL57 2UW, UK
*
*Corresponding author: Department of Parasitology, Cell and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Rahmatieh, Shahrekord, Iran. Tel: 0098 381 2222751. Fax: 0098 381 2221669. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

An enzyme found previously in extracts of adult Schistosoma mansoni worms, that hydrolysed the chromogenic substrate N-acetyl-DL-phenylalanine β-naphthyl-ester, has here been further investigated and characterized. Evidence that the molecule found in the parasite was antigenically and enzymatically homologous with a constituent of normal mouse plasma has been consolidated using a monospecific serum in immunoelectrophoresis and Western immunoblotting. The molecular size of the enzyme was found to be approximately 70 kDa and it was inhibited by a serine protease inhibitor, but not by inhibitors of other classes of protease. The enzymatic activity found in normal mouse serum was also found in normal rat serum, but not in sera from several other mammalian species.

Keywords

schistosomeSchistosoma mansoniproteaseesterasenaphthylamidasemouserathostantigenserumimmune evasion
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 4 , April 2008 , pp. 467 - 472
Copyright
Copyright © Cambridge University Press 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Cioli, D., Knopf, P. M. and Senft, A. W. (1977). Study of Schistosoma mansoni transferred into permissive and nonpermissive hosts. International Journal for Parasitology 7, 293–297.Google Scholar
Curtis, R. H., Fallon, P. G. and Doenhoff, M. J. (1996). Sm480: a high molecular weight Schistosoma mansoni antigen associated with protective immunity. Parasite Immunology 18, 149–157.Google Scholar
Damian, R. T., Greene, N. D. and Hubbard, W. J. (1973). Occurrence of mouse 2-macroglobulin antigenic determinants on Schistosoma mansoni adults, with evidence on their nature. Journal of Parasitology 59, 64–73.Google Scholar
Darani, H. Y., Curtis, R. H. C., McNeice, C., Price, H. P., Sayers, J. R. and Doenhoff, M. J. (1997). Schistosoma mansoni: anomalous immunogenic properties of a 27 kDa larval serine protease associated with protective immunity. Parasitology 115, 237–247.Google Scholar
Dean, D. A. and Sell, K. W. (1972). Surface antigens on Schistosoma mansoni. II. Adsorption of a Forssman-like host antigen by schistosomula. Clinical and Experimental Immunology 12, 525–540.Google Scholar
Dinguirard, N. and Yoshino, T. P. (2006). Potential role of a CD36-like class B scavenger receptor in the binding of modified low-density lipoprotein (acLDL) to the tegumental surface of Schistosoma mansoni sporocysts. Molecular and Biochemical Parasitology 146, 219–230.Google Scholar
Doenhoff, M., Bickle, Q., Long, E., Bain, J. and McGregor, A. (1978). Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. I. Demonstration of resistance to reinfection using a model system that involves perfusion of mice within three weeks of challenge. Journal of Helminthology 52, 173–186.Google Scholar
Doenhoff, M. J., Modha, J., Curtis, R. H. and Adeoye, G. O. (1988). Immunological identification of Schistosoma mansoni peptidases. Molecular and Biochemical Parasitology 31, 233–240.Google Scholar
Dunne, D. W., Agnew, A. M., Modha, J. and Doenhoff, M. J. (1986). Schistosoma mansoni egg antigens: preparation of rabbit antisera with monospecific immunoprecipitating activity, and their use in antigen characterization. Parasite Immunology 8, 575–586.Google Scholar
Furlong, S. T., Thibault, K. S. and Rogers, R. A. (1992). Fluorescent phospholipids preferentially accumulate in sub-tegumental cells of schistosomula of Schistosoma mansoni. Journal of Cell Science 103, 823–830.Google Scholar
Gitter, B. D., McCormick, S. L. and Damian, R. T. (1982). Murine alloantigen acquisition by Schistosoma mansoni: presence of H-2K determinants on adult worms and failure of allogeneic lymphocytes to recognize acquired MHC gene products on schistosomula. Journal of Parasitology 68, 513–518.Google Scholar
Goudie, R. B., Horne, C. H. and Wilkinson, P. C. (1966). A simple method for producing antibody specific to a single selected diffusible antigen. Lancet 2, 1224–1226.Google Scholar
Kemp, W. M., Merritt, S. C., Bogucki, M. S., Rosier, J. G. and Seed, J. R. (1977). Evidence for adsorption of heterospecific host immunoglobulin on the tegument of Schistosoma mansoni. Journal of Immunology 119, 1849–1854.Google Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–685.Google Scholar
Lockwood, B. C., North, M. J., Scott, K. I., Bremner, A. F. and Coombs, G. H. (1987). The use of a highly sensitive electrophoretic method to compare the proteinases of trichomonads. Molecular and Biochemical Parasitology 24, 89–95.Google Scholar
Maizels, R. M., Bundy, D. A., Selkirk, M. E., Smith, D. F. and Anderson, R. M. (1993). Immunological modulation and evasion by helminth parasites in human populations. Nature, London 365, 797–805.Google Scholar
Mancini, G., Carbonara, A. O. and Heremans, J. F. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235–254.Google Scholar
Modha, J., Parikh, V., Gauldie, J. and Doenhoff, M. J. (1988). An association between schistosomes and contrapsin, a mouse serine protease inhibitor (serpin). Parasitology 96, 99–109.Google Scholar
Ramalho-Pinto, F. J. (1987). Decay accelerating factor (DAF) as the host antigen with protective activity to complement killing of schistosomula. Memorias do Instituto Oswaldo Cruz 82 (Suppl 4), 213–216.Google Scholar
Simpson, A. J., Singer, D., McCutchan, T. F., Sacks, D. L. and Sher, A. (1983). Evidence that schistosome MHC antigens are not synthesized by the parasite but are acquired from the host as intact glycoproteins. Journal of Immunology 131, 962–965.Google Scholar
Smithers, S. R. and Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology 55, 695–700.Google Scholar
Smithers, S. R., Terry, R. J. and Hockley, D. J. (1969). Host antigens in schistosomiasis. Proceedings of the Royal Society of London, B 171, 483–494.Google Scholar
Studier, F. W. (1973). Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79, 237–248.Google Scholar
Towbin, H., Staehelin, T. and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4354.Google Scholar
Williams, C. A. and Grabar, P. (1955). Immunoelectrophoretic studies on serum proteins. I. The antigens of human serum. Journal of Immunology 74, 158–168.Google Scholar