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Acetylcholinesterase activity in Nippostrongylus brasiliensis during the course of a primary infection in normal and in protein-deficient rats

Published online by Cambridge University Press:  06 April 2009

Jean Martin
Affiliation:
The Molteno Institute, Downing Street, Cambridge CB2 3EE

Summary

Acetylcholinesterase (AChE) activity was measured in Nippostrongylus brasiliensis recovered during the course of a primary infection in normal and in protein-deficient rats. In well-nourished rats the amount of AChE present in N. brasiliensis increased as the infection progressed, concomitant with the development of the humoral response. In malnourished rats, there was a similar and parallel increase in the amount of AChE synthesized by worms between days 7 and 13 of the infection but as the infection progressed enzyme production decreased so that, by day 26 of the infection, the nematodes contained approximately the same amount of AChE as 8-day-old worms. The possible reasons for this change in enzyme production are discussed.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Africa, C. M. (1931). Studies on the host relations of Nippostrongylus muris with special reference to age resistance and acquired immunity. Journal of Parasitology 18, 113.Google Scholar
Bolin, T. D., Davis, A. E., Cummins, A. G., Duncombe, V. M., & Kelly, J. D., (1977). Effect of iron and protein deficiency on the expulsion of Nippostrongylus brasiliensis from the small intestine of the rat. Gut 18, 182–6.CrossRefGoogle ScholarPubMed
Burt, J. S. & Ogilvie, B. M. (1975). The in vitro maintenance of nematode parasites assayed by acetylcholinesterase and allergen secretion. Experimental Parasitology 38, 7582.CrossRefGoogle ScholarPubMed
Butterworth, A. E. (1977). The eosinophil and its role in immunity to helminth infections. Current Topics in Microbiology and Immunology 77, 127–68.Google Scholar
Clarke, K. R. (1968). The effect of a low protein diet and a glucose and filter paper diet on the course of an infection of Nippostrongylus brasiliensis. Parasitology 58, 325–39.CrossRefGoogle Scholar
Crompton, D. W. T., Arnold, S., Coward, W. A. & Lunn, P. G. (1978). Nippostrongylus (Nematoda) infection in protein-malnourished rats. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, (2) 195–7.Google Scholar
Edwards, A. J., Burt, J S. & Ogilvie, B. M. (1971). The effect of immunity upon some enzymes of the parasitic nematode Nippostrongylus brasiliensis. Parasitology 62, 339–47.CrossRefGoogle ScholarPubMed
Ellman, G. L., Courtney, K. D., Andres, V. & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 7, 8895.CrossRefGoogle ScholarPubMed
Jones, V. E. & Ogilvie, B. M. (1972). Protective immunity to Nippostrongylus brasiliensis in the rat. III; Modulation of worm acetylcholinesterase by antibodies. Immunology 22, 119–29.Google Scholar
Keeling, J. E. D. (1960). The effects of ultra-violet radiation on Nippostrongylus muris 1. Irradiation of infective larvae; lethal and sublethal effects. Annals of Tropical Medicine and Parasitology 54, 182–91.CrossRefGoogle Scholar
Keller, R. & Keist, R. (1972). Protective immunity to Nippostrongylus brasiliensis in the rat. Central role of the lymphocyte in worm expulsion. Immunology 22, 767–73.Google ScholarPubMed
Lee, D. L. (1969). Changes in adult Nippostrongylus brasiliensis during the development of immunity to this nematode in rats. Parasitology 59, 2939.CrossRefGoogle ScholarPubMed
Lee, D. L. (1970). The fine structure of the excretory system in adult Nippostrongylus brasiliensis (Nematoda) and a suggested function for the excretory glands. Tissue and Cell 2, 225–31.CrossRefGoogle Scholar
Ogilvie, B. M. & Hockley, D. J. (1968). Effects of immunity on Nippostrongylus brasiliensis adult worms. Reversible and irreversible changes in infectivity, reproduction and morphology. Journal of Parasitology 54, 1073–84.Google Scholar
Ogilvie, B. M. & Jones, V. E. (1971). Nippostrongylus brasiliensis. A review of immunity and the host/parasite relationship in the rat. Experimental Parasitology 29, 138–77.Google Scholar
Ogilvie, B. M. & Jones, V. E. (1973). Immunity in the parasitic relationship between helminths and hosts. Progress in Allergy 17, 93144.Google ScholarPubMed
Ogilvie, B. M. & Love, R. J. (1974). Cooperation between antibodies and cells in immunity to a nematode parasite. Transplantation Reviews 19, 147–68.Google ScholarPubMed
Ogilvie, B. M., Love, R. J., Jarra, W. & Brown, K. N. (1977). Nippostrongylus brasiliensis in rats: the cellular requirement for worm expulsion. Immunology 32, 521–8.Google ScholarPubMed
Ogilvie, B. M., Rothwell, T. L. W., Bremner, K. C., Schnitzerling, H. J., Nolan, J. & Keith, R. K. (1973). Acetylcholinesterase secretion by parasitic nematodes: 1. Evidence for secretion of the enzyme by a number of nematode species. International Journal for Parasitology 3, 589–97.CrossRefGoogle Scholar
Sanderson, B. E. (1969). Acetylcholinesterase activity in Nippostrongylus brasiliensis (Nematoda). Comparative Biochemistry and Physiology 29, 1207–13.CrossRefGoogle ScholarPubMed
Sanderson, B. E., Jenkins, C. D. & Phillipson, R. F. (1972). Nippostrongylus brasiliensis. Relation between immune damage and acetylcholinesterase levels. International Journal for Parasitology 2, 227–32.CrossRefGoogle ScholarPubMed
Sanderson, B. E. & Ogilvie, B. M. (1971). A study of acetylcholinesterase throughout the life cycle of Nippostrongylus brasiliensis. Parasitology 62, 367–73.CrossRefGoogle ScholarPubMed
Wells, P. D. (1962). Mast cell, eosinophil and histamine levels in Nippostrongylus brasiliensis infected rats. Experimental Parasitology 12, 82101.Google Scholar