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Use of accelerator mass spectrometry for studies in nutrition

Published online by Cambridge University Press:  14 December 2007

George S. Jackson ,
Connie Weaver  and
David Elmore
George S. Jackson
Affiliation:
Department of Physics, Purdue University, West Lafayette, IN 47907, USA
Connie Weaver
Affiliation:
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
David Elmore*
Affiliation:
Department of Physics, Purdue University, West Lafayette, IN 47907, USA
*
*Corresponding author: Professor David Elmore, fax +1 765 496 7228, email [email protected]
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Abstract

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Accelerator mass spectrometry (AMS) is an ultrasensitive analytical technique for measuring rare nuclides such as 14C, 26Al and 41Ca. The low detection limit and wide dynamic range of AMS allow long-term and highly sensitive tracer studies in nutrition that cannot be performed with other methods. The present paper is intended to provide a description of AMS to the interested nutritionist and present proven applications. AMS is compared to liquid scintillation counting and stable isotope MS. A description of common AMS methodology is presented that consists of determining the dose, preparing the sample, diluting the sample (if necessary), and measuring the sample. Applications include Ca metabolism, Al uptake from the environment, dietary intake of carcinogens, fat meta-bolism and folate metabolism. Throughout this discussion the experimental advantages (small doses that pose no health risk, extremely long experimental lifetime, small sample sizes and high sensitivity) made possible by the unique analytical capabilities of AMS are emphasized. The future of AMS is discussed. As the number of AMS centres, instruments, and studies increases, the number of nutritional applications that employ AMS will continue to grow. The coupling of AMS with other analytical techniques (e.g. high performance liquid chromatography) will be developed as access to AMS improves.

Keywords

Accelerator mass spectrometryStable isotopesMicronutrient metabolismFat metabolismCarcinogenRadioisotope
Type
Research Article
Information
Nutrition Research Reviews , Volume 14 , Issue 2 , December 2001 , pp. 317 - 334
Copyright
Copyright © CABI Publishing 2001

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