Tansley Review No. 111 Possible roles of zinc in protecting plant cells from damage by reactive oxygen species

Abstract

Summary 185

I. INTRODUCTION 186

II. EFFECT OF ZINC ON PRODUCTION OF REACTIVE OXYGEN SPECIES 186

1. Superoxide-generating NADPH oxidase 186

2. Zinc deficiency potentiates iron-mediated free radical production 189

(a) Iron accumulation in zinc-deficient plants 189

(b) Iron-induced production of free radicals 189

3. Zinc deficiency-enhanced photooxidation 191

(a) Decrease in photosynthesis 191

(b) Light-induced leaf chlorosis 192

(c) Decrease in indole-3-acetic acid 192

III. MEMBRANE DAMAGE BY REACTIVE OXYGEN SPECIES 193

1. Impairments in membrane structure 193

2. Phospholipids and –SH groups 195

3. Alterations in ion absorption 195

(a) Membrane-bound ATPases 195

(b) Nutrient uptake 197

(c) Changes in activity of ion channels 197

IV. DETOXIFICATION OF REACTIVE OXYGEN SPECIES 198

1. Superoxide dismutases 198

2. H₂O₂-scavenging enzymes 198

V. INVOLVEMENT OF ZINC IN PLANT STRESS TOLERANCE 199

VI. CONCLUSIONS 199

Acknowledgements 200

References 200

Zinc deficiency is one of the most widespread micronutrient deficiencies in plants and causes severe reductions in crop production. There are a number of physiological impairments in Zn-deficient cells causing inhibition of the growth, differentiation and development of plants. Increasing evidence indicates that oxidative damage to critical cell compounds resulting from attack by reactive O₂ species (ROS) is the basis of disturbances in plant growth caused by Zn deficiency. Zinc interferes with membrane-bound NADPH oxidase producing ROS. In Zn-deficient plants the iron concentration increases, which potentiates the production of free radicals. The Zn nutritional status of plants influences photooxidative damage to chloroplasts, catalysed by ROS. Zinc-deficient leaves are highly light-sensitive, rapidly becoming chlorotic and necrotic when exposed to high light intensity. Zinc plays critical roles in the defence system of cells against ROS, and thus represents an excellent protective agent against the oxidation of several vital cell components such as membrane lipids and proteins, chlorophyll, SH-containing enzymes and DNA. The cysteine, histidine and glutamate or aspartate residues represent the most critical Zn- binding sites in enzymes, DNA-binding proteins (Zn-finger proteins) and membrane proteins. In addition, animal studies have shown that Zn is involved in inhibition of apoptosis (programmed cell death) which is preceded by DNA and membrane damage through reactions with ROS.