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Specific PCR-primers as a reliable tool for the detection of white truffles in mycorrhizal roots

Published online by Cambridge University Press:  01 March 1999

ANTONIETTA MELLO ,
LILIA GARNERO  and
PAOLA BONFANTE
ANTONIETTA MELLO
Affiliation:
Centro di Studio sulla Micologia del Terreno del CNR, V. le Mattioli 25, 10125 Torino, Italy
LILIA GARNERO
Affiliation:
Dipartimento di Biologia vegetale dell'Università, V. le Mattioli 25, 10125 Torino, Italy
PAOLA BONFANTE
Affiliation:
Centro di Studio sulla Micologia del Terreno del CNR, V. le Mattioli 25, 10125 Torino, Italy Dipartimento di Biologia vegetale dell'Università, V. le Mattioli 25, 10125 Torino, Italy
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Abstract

During their symbiotic phase, white truffles are barely distinguishable morphologically, and molecular probes are needed for their identification. Here we report the design of species-specific primers for two white truffles (Tuber magnatum and T. borchii) on the basis of their ITS sequence. Their efficiency has been successfully tested on fruit bodies of the same species, many related and unrelated fungal species, as well as mycorrhizal roots in direct, nested and multiplex PCR experiments. They have allowed us to identify T. magnatum mycorrhizas for the first time.

Keywords

Tuber magnatumTuber borchiiPCR-primersnested-PCRmultiplex PCR
Type
Research Article
Information
The New Phytologist , Volume 141 , Issue 3 , March 1999 , pp. 511 - 516
Copyright
© Trustees of New Phytologist 1999

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