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Regulation of thiophene biosynthesis by sulphate in roots of marigolds

Published online by Cambridge University Press:  01 February 1997

R. R. J. ARROO
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
J. J. M. R. JACOBS
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
J. A. M. VAN GESTEL
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
H. KENKEL
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
W. JANNINK
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
A. F. CROES
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
G. J. WULLEMS
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
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Abstract

The incorporation of [35S]sulphur in thiophenes by Tagetes patula roots was used as a model to study the regulation of secondary metabolism with a limited supply of substrate. Growth and thiophene accumulation were measured in root cultures incubated at various sulphate concentrations in the medium. A 20-fold to 40-fold reduction in the sulphate concentration did not affect elongation growth, branching and biomass production within 14 d but decreased the thiophene level to 25–50% of the control in the same period. The reduction in thiophene content was found to result from a decline in biosynthetic capacity of 80–95% after 8 d. This capacity was restored when roots were transferred to standard medium. The restoration took more than 24 h and was suppressed by cordycepin, an inhibitor of mRNA processing. It is concluded that the rate of thiophene synthesis is regulated by a control mechanism that reacts to the availability of sulphate to the roots.

Type
Research Article
Copyright
Trustees of the New Phytologist 1997

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