Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-08T02:39:10.231Z Has data issue: false hasContentIssue false

Regulation of thiophene biosynthesis by sulphate in roots of marigolds

Published online by Cambridge University Press:  01 February 1997

R. R. J. ARROO
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
J. J. M. R. JACOBS
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
J. A. M. VAN GESTEL
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
H. KENKEL
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
W. JANNINK
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
A. F. CROES
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
G. J. WULLEMS
Affiliation:
Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands
Get access

Abstract

The incorporation of [35S]sulphur in thiophenes by Tagetes patula roots was used as a model to study the regulation of secondary metabolism with a limited supply of substrate. Growth and thiophene accumulation were measured in root cultures incubated at various sulphate concentrations in the medium. A 20-fold to 40-fold reduction in the sulphate concentration did not affect elongation growth, branching and biomass production within 14 d but decreased the thiophene level to 25–50% of the control in the same period. The reduction in thiophene content was found to result from a decline in biosynthetic capacity of 80–95% after 8 d. This capacity was restored when roots were transferred to standard medium. The restoration took more than 24 h and was suppressed by cordycepin, an inhibitor of mRNA processing. It is concluded that the rate of thiophene synthesis is regulated by a control mechanism that reacts to the availability of sulphate to the roots.

Type
Research Article
Copyright
Trustees of the New Phytologist 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)