Physiological impacts of Mg deficiency in Pinus radiata: growth and photosynthesis

Abstract

This paper reports the effects of nutrient magnesium (Mg) concentrations on the growth and photosynthetic physiology of clonal Pinus radiata from four female parents (families) known to differ in their tolerance to Mg deficiency and in their needle Mg concentrations. Plants were grown in flowing nutrient solutions with 2 mg l⁻¹ (control) and 0.8 mg l⁻¹ (low) Mg. Plant growth, needle Mg concentration, photosynthesis, chlorophyll fluorescence and carotenoid pigment content were measured. At low Mg, needle Mg concentration was about half that of control plants, height growth was reduced 15–25%, and the needles showed strong visual characteristics of Mg deficiency. Photosynthesis was also halved, and was associated with closure of the stomata under low Mg and with reductions in the residual conductance. In needles from plants grown at low Mg, photochemical yield was reduced both in the light and in the dark, and was strongly dependent on needle Mg concentrations below a threshold concentration of 0.02–0.025% (d. wt basis). The electron transport rate (ETR) at saturating photon flux density in low-Mg-grown needles was reduced to about half that of their Mg controls, but the photon efficiency of ETR was unaffected by the Mg concentration the plants were grown in. Photosynthetic quenching was markedly reduced and non-photosynthetic quenching was increased following growth in low Mg. Growth under low Mg also increased levels of zeaxanthin. Although family differences in growth and photosynthetic physiology were present, few family × Mg interactions were significant. We conclude that Mg deficiency probably affects growth through severe reductions in photosynthesis.