Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-27T21:24:33.080Z Has data issue: false hasContentIssue false

Genetic variability in intergenic spacers of ribosomal DNA in Pisolithus isolates associated with pine, eucalyptus and Afzelia in lowland Kenyan forests

Published online by Cambridge University Press:  01 June 1998

FRANCIS MARTIN
Affiliation:
Equipe de Microbiologie Forestière, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, F-54280 Champenoux, France
CHRISTINE DELARUELLE
Affiliation:
Equipe de Microbiologie Forestière, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, F-54280 Champenoux, France
MIKE IVORY
Affiliation:
Queensland Forestry Research, Institute, 80 Meier's Road, Indooroopilly, Queensland 4068, Australia
Get access

Abstract

Basidiocarps of Pisolithus associated with indigenous (Afzelia quanzensis Welw.) and introduced (Pinus caribaea Mor. and Eucalyptus camaldulensis Dehnh.) hosts in the lowland forests of the Coast Province of Kenya are morphologically distinct. Genetic variability among 52 Pisolithus basidiocarps, collected beneath the various host plants, was examined based on sequence polymorphism within the internal transcribed spacer (ITS) and intergenic spacer (IGS1) of ribosomal DNA genes. Variability in ITS and IGS1 sequences indicated that the three host-associated morphotypes were genetically different. Consensus trees generated by bootstrap analysis of sequence data of Pisolithus isolates from Australia and Kenya are polyphyletic and strongly suggest that the three different morphotypes/genotypes present in Kenya represent separate biological species. In addition, our data indicate that little genetic exchange occurs in silva between these species.

Type
Research Article
Copyright
© Trustees of New Phytologist 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)