Antioxidative systems, pigment and protein contents in leaves of adult mediterranean oak species (Quercus pubescens and Q. ilex) with lifetime exposure to elevated CO₂

Abstract

The aim of the present study was to investigate the effects of elevated CO₂ on the antioxidative systems and the contents of pigments, soluble protein and lipid peroxidation in leaves of adult oaks, Quercus pubescens and Quercus ilex, grown at naturally enriched CO₂ concentrations. For this purpose, a field study was conducted at two CO₂ springs in Central Italy. Measurements of the pre-dawn water potentials indicated less drought stress in trees close to CO₂ springs than in those grown at ambient CO₂ concentrations. Most leaf constituents investigated showed significant variability between sampling dates, species and sites. The foliar contents of protein and chlorophylls were not affected in trees grown close to the CO₂ vents compared with those in ambient conditions. Increases in glutathione and other soluble thiols were observed, but these responses might have been caused by a low pollution of the vents with sulphurous gases. At CO₂ vents, glutathione reductase was unaffected, and superoxide dismutase activity was significantly diminished, in both species. Generally, the activities of catalase, guaiacol peroxidase and ascorbate peroxidase as well as the sum of dehydroascorbate and ascorbate were decreased in leaves from trees grown in naturally CO₂-enriched environments compared with those grown at ambient CO₂ concentrations. The reduction in protective enzymes did not result in increased lipid peroxidation, but increased monodehydroascorbate radical reductase and dehydroascorbate reductase activities found in leaves of Q. pubescens suggest that the smaller pool of ascorbate was subjected to higher turnover rates. These data show that changes in leaf physiology persist, even after lifetime exposure to enhanced atmospheric CO₂. The results suggest that the down-regulation of protective systems, which has also previously been found in young trees or seedlings under controlled exposure to elevated CO₂ concentrations, might reflect a realistic response of antioxidative defences in mature trees in a future high-CO₂ world.