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Genetic variability in intergenic spacers of ribosomal DNA in Pisolithus isolates associated with pine, eucalyptus and Afzelia in lowland Kenyan forests

Published online by Cambridge University Press:  01 June 1998

FRANCIS MARTIN ,
CHRISTINE DELARUELLE  and
MIKE IVORY
FRANCIS MARTIN
Affiliation:
Equipe de Microbiologie Forestière, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, F-54280 Champenoux, France
CHRISTINE DELARUELLE
Affiliation:
Equipe de Microbiologie Forestière, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, F-54280 Champenoux, France
MIKE IVORY
Affiliation:
Queensland Forestry Research, Institute, 80 Meier's Road, Indooroopilly, Queensland 4068, Australia
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Abstract

Basidiocarps of Pisolithus associated with indigenous (Afzelia quanzensis Welw.) and introduced (Pinus caribaea Mor. and Eucalyptus camaldulensis Dehnh.) hosts in the lowland forests of the Coast Province of Kenya are morphologically distinct. Genetic variability among 52 Pisolithus basidiocarps, collected beneath the various host plants, was examined based on sequence polymorphism within the internal transcribed spacer (ITS) and intergenic spacer (IGS1) of ribosomal DNA genes. Variability in ITS and IGS1 sequences indicated that the three host-associated morphotypes were genetically different. Consensus trees generated by bootstrap analysis of sequence data of Pisolithus isolates from Australia and Kenya are polyphyletic and strongly suggest that the three different morphotypes/genotypes present in Kenya represent separate biological species. In addition, our data indicate that little genetic exchange occurs in silva between these species.

Keywords

Afzeliaectomycorrhizal funguseucalyptinternal transcribed spacerintergenic spacerpinePisolithusribosomal DNA
Type
Research Article
Information
The New Phytologist , Volume 139 , Issue 2 , June 1998 , pp. 341 - 352
Copyright
© Trustees of New Phytologist 1998

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