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Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model

Published online by Cambridge University Press:  06 February 2008

Jana Vukovic
Affiliation:
School of Anatomy and Human Biology and Reds Spinal Cord Research Laboratory, The University of Western Australia, Crawley, Australia
Giles W. Plant
Affiliation:
School of Anatomy and Human Biology and Reds Spinal Cord Research Laboratory, The University of Western Australia, Crawley, Australia
Marc J. Ruitenberg
Affiliation:
School of Anatomy and Human Biology and Reds Spinal Cord Research Laboratory, The University of Western Australia, Crawley, Australia
Alan R. Harvey*
Affiliation:
School of Anatomy and Human Biology and Reds Spinal Cord Research Laboratory, The University of Western Australia, Crawley, Australia
*
Correspondence should be addressed to: Professor Alan R. Harvey, School of Anatomy and Human Biology M309, The University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia phone: +61 08 6488 3294 fax: +61 08 6488 1051 email: [email protected]

Abstract

We used an in vivo transplant approach to examine how adult Schwann cells and olfactory ensheathing glia OEG influence the specificity of axontarget cell interactions when they are introduced into the CNS. Populations of either Schwann cells or OEG were mixed with dissociated fetal tectal cells presumptive superior colliculus and, after reaggregation, pieces were grafted onto newborn rat superior colliculus. Both glial types were prelabeled with lentiviral vectors encoding green fluorescent protein. Grafts rapidly established fiber connections with the host and retinal projections into cografts were assessed 656 days posttransplantation by injecting cholera toxinB into host eyes. In control rats that received pure dissociatedreaggregated tectal grafts, retinal ganglion cell RGC axons selectively innervated defined target areas, corresponding to the retinorecipient layer in normal superior colliculus. The pattern of RGC axon ingrowth into OEG containing cografts was similar to that in control grafts. However, in Schwann cell cografts there was reduced host retinal input into presumptive target areas and many RGC axons were scattered throughout the neuropil. Given that OEG in cografts had minimal impact on axontarget cell recognition, OEG might be an appropriate cell type for direct transplantation into injured neuropil when attempting to stimulate specific pathway reconstruction.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2008

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